Quantification of self-renewal capacity in single hematopoietic stem cells from normal and Lnk-deficient mice
In: Developmental cell, Jg. 8 (2004-06-08), Heft 6
Online
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Zugriff:
SummaryDespite being a hallmark of hematopoietic stem cells (HSCs), HSC self-renewal has never been quantitatively assessed. Establishment of a clonal and quantitative assay for HSC function permitted demonstration that adult mouse HSCs are significantly heterogeneous in degree of multilineage repopulation and that higher repopulating potential reflects higher self-renewal activity. An HSC with high repopulating potential could regenerate approximately 1000 HSCs, whereas the repopulating activity of regenerated HSCs on average was significantly reduced, indicating extensive but limited self-renewal capacity in HSCs. Comparisons of wild-type mice with mutant mice deficient in the signal adaptor molecule Lnk showed that not only HSC numbers but also the self-renewal capacity of some HSCs are markedly increased when Lnk function is lost. Lnk appears to control HSC numbers by negatively regulating HSC self-renewal signaling.
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Quantification of self-renewal capacity in single hematopoietic stem cells from normal and Lnk-deficient mice
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Autor/in / Beteiligte Person: | Seita, Jun ; Takatsu, Kiyoshi ; Matsubara, Azusa ; Nakauchi, Hiromitsu ; Osawa, Mitsujiro ; Ema, Hideo ; Morita, Yohei ; Sudo, Kazuhiro ; Takaki, Satoshi |
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Zeitschrift: | Developmental cell, Jg. 8 (2004-06-08), Heft 6 |
Veröffentlichung: | 2004 |
Medientyp: | unknown |
ISSN: | 1534-5807 (print) |
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