Characterization of forsythoside A metabolites in rats by a combination of UHPLC-LTQ-Orbitrap mass spectrometer with multiple data processing techniques
In: Biomedical chromatography : BMC, Jg. 32 (2017-08-29), Heft 5
Online
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Zugriff:
Forsythoside A (FTA), the main active constituent isolated from Fructus Forsythiae, has various biological functions including anti-oxidant, anti-viral and anti-microbial activities. However, while research on FTA has been mainly focused on the treatment of diseases on a material basis, FTA metabolites in vivo have not been comprehensively evaluated. Here, a rapid and sensitive method using a UHPLC-LTQ-Orbitrap mass spectrometer with multiple data processing techniques including high-resolution extracted ion chromatograms, multiple mass defect filters and diagnostic product ions was developed for the screening and identification of FTA metabolites in rats. As the result, a total of 43 metabolites were identified in biological samples including 42 metabolites in urine, 22 metabolites in plasma and 15 metabolites in feces. These results demonstrated that FTA underwent a series of in vivo metabolic reactions including methylation, dimethylation, sulfation, glucuronidation, diglucuronidation, cysteine conjugation and their composite reactions. The research enhanced our understanding of FTA metabolism and built a foundation for further toxicity and safety studies.
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Characterization of forsythoside A metabolites in rats by a combination of UHPLC-LTQ-Orbitrap mass spectrometer with multiple data processing techniques
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Autor/in / Beteiligte Person: | Xu, Lu-Lu ; Zhang, Jia-Yu ; Cao, Guang-shang ; Liu, Ying ; Wang, Fei ; Li, Yun ; Wang, Zhibin ; Lu, Jian-Qiu |
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Zeitschrift: | Biomedical chromatography : BMC, Jg. 32 (2017-08-29), Heft 5 |
Veröffentlichung: | 2017 |
Medientyp: | unknown |
ISSN: | 1099-0801 (print) |
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