Effects of growth factors and trefoil peptides on migration and replication in primary oxyntic cultures
In: The American journal of physiology, Jg. 276 (1999-05-18), Heft 5
Online
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Zugriff:
Restitution, the lateral migration of cells over an intact basement membrane, maintains mucosal integrity. We studied the regulation of migration and proliferation of enzyme-dispersed canine oxyntic mucosa cells in primary culture. Confluent monolayers were wounded and cultured in serum-free medium, and cells migrating into the wound were counted. [3H]thymidine incorporation into DNA was studied using subconfluent cultures. Considerable migration occurred in untreated monolayers; however, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I), two trefoil peptides, and interleukin (IL)-1beta further enhanced migration. The specific EGF receptor (EGFR) monoclonal antibody, MAb-528, inhibited both basal and TGF-alpha- or IL-1beta-stimulated migration, but not the response to trefoil peptide, bFGF, or IGF-I. Exogenous TGF-beta inhibited cell proliferation but did not alter migration. Immunoneutralization with anti-TGF-beta blocked the response to exogenous TGF-beta and produced a small enhancement of basal thymidine incorporation but did not attenuate basal or TGF-alpha-stimulated migration. In conclusion, endogenous EGFR ligands regulate proliferation and migration. TGF-beta inhibits mitogenesis; it did not upregulate migration in these cultures. Although bFGF, IGF-I, and IL-1beta enhance gastric epithelial migration, only IL-1beta acted in a TGF-alpha-dependent fashion.
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Effects of growth factors and trefoil peptides on migration and replication in primary oxyntic cultures
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Autor/in / Beteiligte Person: | Chen, Monica C. ; Soll, Andrew H. ; Minh Tan Nguyen ; Podolsky, Daniel K. ; Frank Serge Lehmann ; Kato, Kimitoshi |
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Zeitschrift: | The American journal of physiology, Jg. 276 (1999-05-18), Heft 5 |
Veröffentlichung: | 1999 |
Medientyp: | unknown |
ISSN: | 0002-9513 (print) |
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