Methanol production by reversed methylotrophy constructed in Escherichia coli
In: Bioscience, Biotechnology, and Biochemistry, Jg. 84 (2020-05-03), S. 1062-1068
Online
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Zugriff:
We constructed a reversed methylotrophic pathway that produces methanol, a promising feedstock for production of useful compounds, from fructose 6-phosphate (F6P), which can be supplied by catabolism of biomass-derived sugars including glucose, by a synthetic biology approach. Using Escherichia coli as an expression host, we heterologously expressed genes encoding methanol utilization enzymes from methylotrophic bacteria, i.e. the NAD+-dependent methanol dehydrogenase (MDH) from Bacillus methanolicus S1 and an artificial fusion enzyme of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase from Mycobacterium gastri MB19 (HPS-PHI). We confirmed that these enzymes can catalyze reverse reactions of methanol oxidation and formaldehyde fixation. The engineered E. coli strain co-expressing MDH and HPS-PHI genes produced methanol in resting cell reactions not only from F6P but also from glucose. We successfully conferred reversed methylotrophy to E. coli and our results provide a proof-of-concept for biological methanol production from biomass-derived sugar compounds. Reversed methylotrophy to produce methanol from glucose or F6P was constructed by introducing MDH and HPS-PHI into E. coli cells.
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Methanol production by reversed methylotrophy constructed in Escherichia coli
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Autor/in / Beteiligte Person: | Yamakita, Miyabi ; Yurimoto, Hiroya ; Hayashi, Daisuke ; Sakai, Yasuyoshi ; Fujisawa, Kento ; Takeya, Tomoyuki |
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Zeitschrift: | Bioscience, Biotechnology, and Biochemistry, Jg. 84 (2020-05-03), S. 1062-1068 |
Veröffentlichung: | Informa UK Limited, 2020 |
Medientyp: | unknown |
ISSN: | 1347-6947 (print) ; 0916-8451 (print) |
DOI: | 10.1080/09168451.2020.1715202 |
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