Mycobacterium bovis Requires P27 (LprG) To Arrest Phagosome Maturation and Replicate within Bovine Macrophages

Federico Carlos Blanco ; Gutierrez, Maximiliano G. ; et al.

In: Infection and immunity, Jg. 85 (2016-08-16), Heft 3

Online unknown

Zugriff:

Mycobacterium bovis causes tuberculosis in a wide variety of mammals, with strong tropism for cattle and eventually humans. P27, also called LprG, is among the proteins involved in the mechanisms of the virulence and persistence of M. bovis and Mycobacterium tuberculosis. Here, we describe a novel function of P27 in the interaction of M. bovis with its natural host cell, the bovine macrophage. We found that a deletion in the p27-p55 operon impairs the replication of M. bovis in bovine macrophages. Importantly, we show for the first time that M. bovis arrests phagosome maturation in a process that depends on P27. This effect is P27 specific since complementation with wild-type p27 but not p55 fully restored the wild-type phenotype of the mutant strain; this indicates that P55 plays no important role during the early events of M. bovis infection. In addition, we also showed that the presence of P27 from M. smegmatis decreases the association of LAMP-3 with bead phagosomes, indicating that P27 itself blocks phagosome-lysosome fusion by modulating the traffic machinery in the cell host. Fil: Vázquez, Cristina Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Bianco, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gutierrez, Maximiliano Gabriel. The Francis Crick Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina

Titel:	Mycobacterium bovis Requires P27 (LprG) To Arrest Phagosome Maturation and Replicate within Bovine Macrophages
Autor/in / Beteiligte Person:	Federico Carlos Blanco ; Gutierrez, Maximiliano G. ; Cristina Lourdes Vázquez ; Marina Andrea Forrellad ; María Verónica Bianco ; Bigi, Fabiana
Link:	View record in OpenAIRE (Volltext) https://pubmed.ncbi.nlm.nih.gov/28031264
Zeitschrift:	Infection and immunity, Jg. 85 (2016-08-16), Heft 3
Veröffentlichung:	2016
Medientyp:	unknown
ISSN:	1098-5522 (print)
Schlagwort:	0301 basic medicine Lipoproteins 030106 microbiology Immunology Virulence Gene Expression Nitric Oxide Synthase Type II P27 Enfermedades de los Animales MYCOBACTERIUM BOVIS Microbiology Animal Diseases purl.org/becyt/ford/1 [https] Ciencias Biológicas Mycobacterium tuberculosis 03 medical and health sciences Biología Celular, Microbiología Phagosomes Phagosome maturation Operon Tuberculosis Macrophage Animals Humans purl.org/becyt/ford/1.6 [https] Macrofagos Tropism Phagosome Mycobacterium bovis Microbial Viability Cellular Microbiology: Pathogen-Host Cell Molecular Interactions biology Macrophages Cell Cycle Checkpoints biology.organism_classification 3. Good health Complementation 030104 developmental biology Infectious Diseases LprG Ganado Bovino Mutation LPRG Parasitology Cattle CIENCIAS NATURALES Y EXACTAS HeLa Cells
Sonstiges:	Nachgewiesen in: OpenAIRE File Description: application/pdf Rights: OPEN

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