Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts
In: Lasers in medical science, Jg. 24 (2007-11-09), Heft 6
Online
unknown
Zugriff:
The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium-aluminum-arsenium (GaAlAs), 780 nm, or indium-gallium-aluminum-phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm(2), 3 J/cm(2) and 5 J/cm(2) (3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey's test (P/= 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.
Titel: |
Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts
|
---|---|
Autor/in / Beteiligte Person: | Sueli Patricia Harumi Miyagi ; Carla Andreotti Damante ; Giorgio De Micheli ; Márcia Martins Marques ; Ilíria Salomão Feist |
Link: | |
Zeitschrift: | Lasers in medical science, Jg. 24 (2007-11-09), Heft 6 |
Veröffentlichung: | 2007 |
Medientyp: | unknown |
ISSN: | 1435-604X (print) |
Schlagwort: |
|
Sonstiges: |
|