Mulberry 1-Deoxynojirimycin Inhibits Adipogenesis by Repression of the ERK/PPARγ Signaling Pathway in Porcine Intramuscular Adipocytes
In: Journal of Agricultural and Food Chemistry, Jg. 63 (2015-06-25), S. 6212-6220
Online
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Zugriff:
Intramuscular fat (IMF), which is modulated by adipogenensis of intramuscular adipocytes, plays a key role in pork quality associated with marbling, juiceness, and flavor. However, the regulatory mechanism of 1-deoxynojirimycin (DNJ) on adipogenesis is still unknown. Here, we found that both DNJ (2.0, 3.0, 4.0, 5.0, and 6.0 μM) and rosiglitazone (RSG; 0.1, 0.2, 0.3, 0.4, and 0.5 mM) had no effect on cell viability. Moreover, 4 μM DNJ significantly inhibited adipogenesis, whereas 0.4 mM RSG increased lipogenesis of porcine intramuscular adipocytes. Interestingly, DNJ sharply inhibited phosphorylation of extracellular regulated protein kinases 1/2 (ERK1/2), but did not change phosphorylation of AKT (protein kinase B) in intramuscular adipocytes. We further found that the inhibitory adipogenesis of DNJ was attenuated by RSG via up-regulation of PPARγ. On the basis of the above findings, we suggest that DNJ inhibited adipogenesis through the ERK/PPARγ signaling pathway in porcine intramuscular adipocytes.
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Mulberry 1-Deoxynojirimycin Inhibits Adipogenesis by Repression of the ERK/PPARγ Signaling Pathway in Porcine Intramuscular Adipocytes
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Autor/in / Beteiligte Person: | Pang, Weijun ; Ma, Meilin ; Chen, Xiaochang ; Gao, Yun ; Wang, Guoqiang ; Yu, Taiyong ; Yang, Gongshe ; Zhu, Li |
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Zeitschrift: | Journal of Agricultural and Food Chemistry, Jg. 63 (2015-06-25), S. 6212-6220 |
Veröffentlichung: | American Chemical Society (ACS), 2015 |
Medientyp: | unknown |
ISSN: | 1520-5118 (print) ; 0021-8561 (print) |
DOI: | 10.1021/acs.jafc.5b01680 |
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