Quantitative screening for cyanotoxins in soil and groundwater of agricultural watersheds in Quebec, Canada

Cyanotoxins, as secondary metabolites of cyanobacteria, are highly toxic to humans, animals and plants. Cyanobacterial blooms are 'hot spots' for cyanotoxin production, but we hypothesized that cyanotoxins will be present in multiple ecological compartments of agricultural watersheds. We detected cyanotoxins in the vadose zone (soil and drainage water from the soil profile) and in groundwater used as a drinking water source from agricultural watersheds. Cyanotoxins detection was confirmed with enzyme-linked immunosorbent assay kits and ultra-high liquid chromatography with tandem mass spectrometry. This work confirms that cyanotoxins exist outside of freshwater lakes, based on detection of microcystins in the vadose zone and in drinking water sourced from groundwater in agricultural watersheds. This suggests that cyanotoxins may be transferred from cyanobacterial blooms in lakes to groundwater through normal hydrologic processes. Public health authorities should be alerted to cyanotoxins in drinking water supplies and proper monitoring and treatment protocols should be implemented to protect citizens from this potential health hazard.