Epitope presentation system based on cucumber mosaic virus coat protein expressed from a potato virus X-based vector
In: Archives of Virology, Jg. 151 (2006-02-20), S. 1373-1386
Online
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Zugriff:
The Cucumber mosaic virus Ixora isolate (CMV) coat protein gene (CP) was placed under the transcriptional control of the duplicated subgenomic CP promoter of a Potato virus X (PVX)-based vector. In vitro RNA transcripts were inoculated onto Nicotiana benthamiana plants and recombinant CMV capsid proteins were identified on Western blots probed with CMV antibodies 5-7 days post-inoculation. PVX-produced CMV CP subunits were capable of assembling into virus-like particles (VLPs), which were visualized by electron microscopy. We further used the PVX/CMVCP system for transient expression of recombinant CMV CP constructs containing different neutralizing epitopes of Newcastle disease virus (NDV) engineered into the internal betaH-betaI (motif 5) loop. Both crude plant extracts and purified VLPs were immunoreactive with CMV antibodies as well as with epitope-specific antibodies to NDV, thus confirming the surface display of the engineered NDV epitope. Our study demonstrates the potential of PVX/CMVCP as an expression tool and as a presentation system for promising epitopes.
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Epitope presentation system based on cucumber mosaic virus coat protein expressed from a potato virus X-based vector
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Autor/in / Beteiligte Person: | Nemchinov, Lev G. ; Hammond, Rosemarie W. ; Natilla, A. |
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Zeitschrift: | Archives of Virology, Jg. 151 (2006-02-20), S. 1373-1386 |
Veröffentlichung: | Springer Science and Business Media LLC, 2006 |
Medientyp: | unknown |
ISSN: | 1432-8798 (print) ; 0304-8608 (print) |
DOI: | 10.1007/s00705-005-0711-x |
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