Biochemical Analysis of the Yeast Proteinase Inhibitor (IC) Homolog ICh and Its Comparison with IC

Kato, Michiko ; Fukada, Hiroaki ; et al.

In: Bioscience, Biotechnology, and Biochemistry, Jg. 71 (2007-02-23), S. 472-480

Online unknown

Zugriff:

Carboxypeptidase Y (CPY) inhibitor (I(C)) and its homologous protein (I(C)h) are thought to be members of the phosphatidylethanolamine-binding protein (PEBP) family of Saccharomyces cerevisiae. The biochemical characterization of I(C) and its inhibition mode toward CPY were recently reported, but I(C)h has not been characterized. The molecular mass of I(C)h was determined to be 22,033.7. The N-terminal Met1 was cleaved and the amino group of Ser2 was acetylated. I(C)h is folded as a monomeric beta-protein and is devoid of disulfide bonds. It has no inhibitory activity toward CPY, and it does not form a complex with CPY. I(C)h was exclusively expressed in the early log phase, whereas I(C) was expressed in the logarithmic and stationary phase. The intracellular localization of I(C)h was different from that of I(C). These findings provide insights into the physiological functions of I(C)h.

Titel:	Biochemical Analysis of the Yeast Proteinase Inhibitor (IC) Homolog ICh and Its Comparison with IC
Autor/in / Beteiligte Person:	Kato, Michiko ; Fukada, Hiroaki ; Nagayama, Mitsuru ; Mima, Joji ; Ueda, Mitsuyoshi
Link:	View record in OpenAIRE (Volltext) https://doi.org/10.1271/bbb.60528
Zeitschrift:	Bioscience, Biotechnology, and Biochemistry, Jg. 71 (2007-02-23), S. 472-480
Veröffentlichung:	Oxford University Press (OUP), 2007
Medientyp:	unknown
ISSN:	1347-6947 (print) ; 0916-8451 (print)
DOI:	10.1271/bbb.60528
Schlagwort:	Saccharomyces cerevisiae Proteins Molecular Sequence Data Saccharomyces cerevisiae Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Amino Acid Sequence cardiovascular diseases Molecular Biology chemistry.chemical_classification Phosphatidylethanolamine biology Molecular mass Circular Dichroism Binding protein Sulfhydryl Reagents Organic Chemistry Intracellular Signaling Peptides and Proteins General Medicine Protein superfamily Blotting, Northern biology.organism_classification Molecular biology Yeast nervous system diseases Molecular Weight Enzyme chemistry Acetylation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Chromatography, Gel Spectrophotometry, Ultraviolet Carrier Proteins Protein Processing, Post-Translational Biotechnology
Sonstiges:	Nachgewiesen in: OpenAIRE

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