Comparative ability of various plasmid-based cytokines and chemokines to adjuvant the activity of HIV plasmid DNA vaccines
In: Vaccine, Jg. 26 (2008-05-13), Heft 37
Online
unknown
Zugriff:
The effectiveness of plasmid DNA (pDNA) vaccines can be improved by the co-delivery of plasmid-encoded molecular adjuvants. We evaluated pDNAs encoding GM-CSF, Flt-3L, IL-12 alone, or in combination, for their relative ability to serve as adjuvants to augment humoral and cell-mediated immune responses elicited by prototype pDNA vaccines. In Balb/c mice we found that co-administration of plasmid-based murine GM-CSF (pmGM-CSF), murine Flt-3L (pmFlt-3L) or murine IL-12 (pmIL-12) could markedly enhance the cell-mediated immune response elicited by an HIV-1 env pDNA vaccine. Plasmid mGM-CSF also augmented the immune response elicited by DNA vaccines expressing HIV-1 Gag and Nef-Tat-Vif. In addition, the use of pmGM-CSF as a vaccine adjuvant appeared to markedly increase antigen-specific proliferative responses and improved the quality of the resulting T cell response by increasing the percentage of poly-functional memory CD8(+) T cells. Co-delivery of pmFlt-3L with pmGM-CSF did not result in a further increase in adjuvant activity. However, the co-administration of pmGM-CSF with pmIL-12 did significantly enhanced Env-specific proliferative responses and vaccine efficacy in the murine vaccinia virus challenge model relative to mice immunized with the env pDNA vaccine adjuvanted with either pmGM-CSF or pmIL-12 alone. These data support the testing of pmGM-CSF and pmIL-12, used alone or in combination, as plasmid DNA vaccine adjuvants in future macaque challenge studies.
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Comparative ability of various plasmid-based cytokines and chemokines to adjuvant the activity of HIV plasmid DNA vaccines
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Autor/in / Beteiligte Person: | Megati, Shakuntala ; Eldridge, John H. ; Sidhu, Maninder K. ; Xu, Rong ; Roopchand, Vidia ; Weiner, David B. ; Luckay, Amara ; Felber, Barbara K. ; Masood, Amjed ; Garcia-Hand, Dorys ; Rosati, Margherita ; Egan, Michael A. ; Pavlakis, George N. |
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Zeitschrift: | Vaccine, Jg. 26 (2008-05-13), Heft 37 |
Veröffentlichung: | 2008 |
Medientyp: | unknown |
ISSN: | 0264-410X (print) |
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