Coordination of mRNA and tRNA methylations by TRMT10A
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 117 (2020-03-28), Heft 14
Online
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Zugriff:
The posttranscriptional modification of messenger RNA (mRNA) and transfer RNA (tRNA) provides an additional layer of regulatory complexity during gene expression. Here, we show that a tRNA methyltransferase, TRMT10A, interacts with an mRNA demethylase FTO (ALKBH9), both in vitro and inside cells. TRMT10A installs N 1 -methylguanosine (m 1 G) in tRNA, and FTO performs demethylation on N 6 -methyladenosine (m 6 A) and N 6 ,2′- O -dimethyladenosine (m 6 A m ) in mRNA. We show that TRMT10A ablation not only leads to decreased m 1 G in tRNA but also significantly increases m 6 A levels in mRNA. Cross-linking and immunoprecipitation, followed by high-throughput sequencing results show that TRMT10A shares a significant overlap of associated mRNAs with FTO, and these mRNAs have accelerated decay rates potentially through the regulation by a specific m 6 A reader, YTHDF2. Furthermore, transcripts with increased m 6 A upon TRMT10A ablation contain an overrepresentation of m 1 G9-containing tRNAs codons read by tRNA Gln(TTG) , tRNA Arg(CCG) , and tRNA Thr(CGT) . These findings collectively reveal the presence of coordinated mRNA and tRNA methylations and demonstrate a mechanism for regulating gene expression through the interactions between mRNA and tRNA modifying enzymes.
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Coordination of mRNA and tRNA methylations by TRMT10A
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Autor/in / Beteiligte Person: | Stoute, Julian ; Cui, Yixiao ; Kathy Fange Liu ; Zhang, Yuyu ; Shen, Hui ; Yanas, Amber ; R. Jordan Ontiveros |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 117 (2020-03-28), Heft 14 |
Veröffentlichung: | 2020 |
Medientyp: | unknown |
ISSN: | 1091-6490 (print) |
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