The Nucleocapsid protein triggers the main humoral immune response in COVID-19 patients
In: Biochemical and Biophysical Research Communications, Jg. 543 (2021-03-01), S. 45-49
Online
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Zugriff:
In order to control the COVID-19 pandemic caused by SARS-CoV-2 infection, serious progress has been made to identify infected patients and to detect patients with a positive immune response against the virus. Currently, attempts to generate a vaccine against the coronavirus are ongoing. To understand SARS-CoV-2 immunoreactivity, we compared the IgG antibody response against SARS-CoV-2 in infected versus control patients by dot blot using recombinant viral particle proteins: N (Nucleocapsid), M (Membrane) and S (Spike). In addition, we used different protein fragments of the N and S protein to map immune epitopes. Most of the COVID-19 patients presented a specific immune response against the full length and fragments of the N protein and, to lesser extent, against a fragment containing amino acids 300–685 of the S protein. In contrast, immunoreactivity against other S protein fragments or the M protein was low. This response is specific for COVID-19 patients as very few of the control patients displayed immunoreactivity, likely reflecting an immune response against other coronaviruses. Altogether, our results may help develop method(s) for measuring COVID-19 antibody response, selectivity of methods detecting such SARS-CoV-2 antibodies and vaccine development.
Highlights • COVID-19 patients present a specific immune response against all regions of SARS-CoV-2 N protein. • COVID-19 patients also respond to a fragment containing amino acids 300–685 of the S protein. • Immunoreactivity against other S protein fragments or the M protein is low in COVID-19 patients. • Very few of the control patients display immunoreactivity against SARS-CoV-2 proteins.
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The Nucleocapsid protein triggers the main humoral immune response in COVID-19 patients
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Autor/in / Beteiligte Person: | Hernández-Carralero, Esperanza ; Hernández-Reyes, Yeray ; Cabrera, Elisa ; Maria Cristina Paz-Cabrera ; Freire, Raimundo ; Hernández-Porto, Miriam ; Smits, Veronique A. J. ; Salido, Eduardo ; Juan Ramon Hernandez-Fernaud ; Gillespie, David A. |
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Zeitschrift: | Biochemical and Biophysical Research Communications, Jg. 543 (2021-03-01), S. 45-49 |
Veröffentlichung: | Elsevier BV, 2021 |
Medientyp: | unknown |
ISSN: | 0006-291X (print) |
DOI: | 10.1016/j.bbrc.2021.01.073 |
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