A novel human CD32 mAb blocks experimental immune haemolytic anaemia in FcgammaRIIA transgenic mice
In: British journal of haematology, Jg. 130 (2005-06-29), Heft 1
Online
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Zugriff:
A fully human IgG1 kappa antibody (MDE-8) was generated, which recognised Fc-gamma receptor IIa (FcgammaRIIa) molecules on CD32 transfectants, peripheral blood monocytes, polymorphonuclear cells and platelets. This antibody blocked FcgammaRIIa ligand-binding via its F(ab')(2) fragment. Overnight incubation of monocytes with F(ab')(2) fragments of MDE-8 leads to a c. 60% decrease in cell surface expression of FcgammaRIIa. MDE-8 whole antibody induced a concomitant c. 30% decrease of FcgammaRI on THP-1 cells and monocytes. In humans FcgammaRIIa plays an important role in the clearance of antibody-coated red blood cells in vivo. As an equivalent of FcgammaRIIa does not exist in mice, the in vivo effect of MDE-8 was studied in an FcgammaRIIa transgenic mouse model. In these mice, antibody-induced anaemia could readily be blocked by MDE-8. These data document a new human antibody that effectively blocks FcgammaRIIa, induces modulation of both FcgammaRIIa and FcgammaRI from phagocytic cells, and ameliorates antibody-induced anaemia in vivo.
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A novel human CD32 mAb blocks experimental immune haemolytic anaemia in FcgammaRIIA transgenic mice
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Autor/in / Beteiligte Person: | Walraven, Vanessa ; Leusen, Jeanette H. W. ; Gerritsen, Arnout F. ; Annet van Royen-Kerkhof ; Teeling, Jessica L. ; Sanders, Elisabeth A. M. ; Rijkers, Ger T. ; Saeland, Eirikur ; Vitale, Laura ; Voorhorst-Ogink, Marleen M. ; Marc A. van Dijk ; Kuis, Wietse ; McKenzie, Steven E. ; Keler, Tibor ; Jan G. J. van de Winkel |
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Zeitschrift: | British journal of haematology, Jg. 130 (2005-06-29), Heft 1 |
Veröffentlichung: | 2005 |
Medientyp: | unknown |
ISSN: | 0007-1048 (print) |
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