Optimizing the Thermal Read-Out Technique for MIP-Based Biomimetic Sensors: Towards Nanomolar Detection Limits
In: Sensors, Jg. 13 (2013), Heft 7, S. 9148-9159
Online
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Zugriff:
In previous work, the novel heat-transfer method (HTM) for the detection of small molecules with Molecularly Imprinted Polymers (MIP)-type receptors was presented. In this study we focus on optimization of this sensor performance, with as final aim to lower the detection limit by reducing the noise level. It was determined that the noise originates foremost from the power supply, which can be controlled by varying the PID parameters. Therefore, the effect of the individual parameters was evaluated by tuning P, I and D separately at a temperature of 37 degrees C, giving a first indication of the optimal configuration. Next, a temperature profile was programmed and the standard deviation of the heat-transfer resistance over the entire regime was studied for a set of parameters. The optimal configuration, P1-I6-D0, reduced the noise level with nearly a factor of three compared to the original parameters of P10-I5-D0. With the optimized settings, the detection of L-nicotine in buffer solutions was studied and the detection limit improved significantly from 100 nM to 35 nM. Summarizing, optimization of the PID parameters and thereby improving the detection limit is a key parameter for first applications of the HTM-method for MIP receptors in analytical research. This work is supported by the Life-Science Initiative of the Province of Limburg, by the Special Research Funds of Hasselt University, the Methusalem Nano Antwerp-Hasselt, the European Funds for Regional Development-MicroBioMed and the Research Foundation Flanders FWO project G.0B62.13N (Exploration of heat-transfer effects for bio- and chemosensors). The authors also would like to thank Thomas Cleij, Huguette Penxten, Johan Soogen, Christel Willems, Johnny Baccus, Lieven De Winter, Jan Mertens, and Andries Van Genechten for technical assistance and stimulating scientific discussions.
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Optimizing the Thermal Read-Out Technique for MIP-Based Biomimetic Sensors: Towards Nanomolar Detection Limits
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Autor/in / Beteiligte Person: | Bart van Grinsven ; Wagner, Patrick ; Ward De Ceuninck ; Geerets, Bram ; Peeters, Marloes ; Bers, Karolien |
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Zeitschrift: | Sensors, Jg. 13 (2013), Heft 7, S. 9148-9159 |
Veröffentlichung: | MDPI AG, 2013 |
Medientyp: | unknown |
ISSN: | 1424-8220 (print) |
DOI: | 10.3390/s130709148 |
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