Microbiota of Severe Early Childhood Caries before and after Therapy
In: Journal of Dental Research, Jg. 90 (2011-08-25), S. 1298-1305
Online
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Zugriff:
Severe early childhood caries (ECC) is difficult to treat successfully. This study aimed to characterize the microbiota of severe ECC and evaluate whether baseline or follow-up microbiotas are associated with new lesions post-treatment. Plaque samples from 2- to 6-year-old children were analyzed by a 16S rRNA-based microarray and by PCR for selected taxa. Severe-ECC children were monitored for 12 months post-therapy. By microarray, species associated with severe-ECC (n = 53) compared with caries-free (n = 32) children included Slackia exigua (p = 0.002), Streptococcus parasanguinis (p = 0.013), and Prevotella species (p < 0.02). By PCR, severe-ECC-associated taxa included Bifidobacteriaceae (p < 0.001), Scardovia wiggsiae (p = 0.003), Streptococcus mutans with bifidobacteria (p < 0.001), and S. mutans with S. wiggsiae (p = 0.001). In follow-up, children without new lesions (n = 36) showed lower detection of taxa including S. mutans, changes not observed in children with follow-up lesions (n = 17). Partial least-squares modeling separated the children into caries-free and two severe-ECC groups with either a stronger bacterial or a stronger dietary component. We conclude that several species, including S. wiggsiae and S. exigua, are associated with the ecology of advanced caries, that successful treatment is accompanied by a change in the microbiota, and that severe ECC is diverse, with influences from selected bacteria or from diet.
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Microbiota of Severe Early Childhood Caries before and after Therapy
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Autor/in / Beteiligte Person: | Hughes, C. V. ; Kent, Ralph ; Anne C.R. Tanner ; P. Lif Holgerson ; Kanasi, Eleni ; Johansson, Ingegerd ; Loo, Cheen Y. ; N I Chalmers |
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Zeitschrift: | Journal of Dental Research, Jg. 90 (2011-08-25), S. 1298-1305 |
Veröffentlichung: | SAGE Publications, 2011 |
Medientyp: | unknown |
ISSN: | 1544-0591 (print) ; 0022-0345 (print) |
DOI: | 10.1177/0022034511421201 |
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