Caffeine-stimulated GTH-II release involves Ca2+stores with novel properties
In: American Journal of Physiology-Cell Physiology, Jg. 282 (2002-03-01), S. C635- (11S.)
Online
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Zugriff:
Modulation of Ca2+stores with 10 mM caffeine stimulates robust secretion of gonadotropin (GTH-II) from goldfish gonadotropes. Although both endogenous forms of gonadotropin-releasing hormone (GnRH) utilize a common intracellular Ca2+store, sGnRH, but not cGnRH-II, uses an additional caffeine-sensitive mechanism. We examined caffeine signaling by using Ca2+imaging, electrophysiology, and cell-column perifusion. Although caffeine inhibited K+channels, this action appeared to be unrelated to caffeine-induced GTH-II release, because the latter was insensitive to tetraethylammonium. The effects of caffeine also were not mediated by the cAMP/protein kinase A pathway. Instead, caffeine-evoked GTH-II responses were Ca2+signal dependent because they were abolished by 1,2-bis(2-aminophenoxy)ethane- N,N,N′,N′-tetraacetic acid loading. Caffeine generated localized Ca2+signals that began near secretory granules. Surprisingly, caffeine-stimulated GTH-II release was insensitive to 100 μM ryanodine and, unlike GnRH action, was unaffected by inhibitors of voltage-gated Ca2+channels or sarco(endo)plasmic reticulum Ca2+-ATPases. Collectively, these data indicate that caffeine-stimulated GTH-II release is not mediated by typical agonist-sensitive Ca2+stores found in endoplasmic reticulum.
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Caffeine-stimulated GTH-II release involves Ca2+stores with novel properties
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Autor/in / Beteiligte Person: | Wong, Calvin J. H. ; Yunker, Warren K. ; Johnson, James D. ; Chang, John P. |
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Zeitschrift: | American Journal of Physiology-Cell Physiology, Jg. 282 (2002-03-01), S. C635- (11S.) |
Veröffentlichung: | American Physiological Society, 2002 |
Medientyp: | unknown |
ISSN: | 1522-1563 (print) ; 0363-6143 (print) |
DOI: | 10.1152/ajpcell.00044.2001 |
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