IEF peptide fractionation method combined to shotgun proteomics enhances the exploration of rice milk proteome
In: Analytical biochemistry, Jg. 537 (2017-06-30)
Online
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Zugriff:
We conducted a proteomics study in order to detect the proteomic method which provides the most complete characterization of the proteins of rice milk. In particular, we compared the results obtained from LC-MS/MS after protein precipitation with acetone or TCA, as well as the results obtained from LC-MS/MS after protein prefractionation based on SDS-PAGE (GeLC-MS/MS) or ProteoMiner™ technology (ProteoMiner-LC-MS/MS), and after peptide prefractionation based on IEF (pIEF-LC-MS/MS). A total of 158 protein species have been detect in rice milk. The physical-chemical analysis and classification of the identified proteins were also reported. In particular, we showed that pIEF-LC-MS/MS method led to a significant increase in the proteome coverage, allowing the identification of a total of 96 proteins of milk rice. This study demonstrates the utility of a prefractionation step based on pIEF before the shotgun proteomic analysis and offers an in-depth insight into the rice milk proteome.
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IEF peptide fractionation method combined to shotgun proteomics enhances the exploration of rice milk proteome
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Autor/in / Beteiligte Person: | Cecconi, Daniela ; Pidutti, Paolo ; Brandi, Jessica ; Gosetti, Fabio ; Marengo, Emilio ; Manfredi, Marcello ; Conte, Eleonora ; Robotti, Elisa ; Manfredi, M ; Brandi, J ; Conte, E ; Pidutti, P ; Gosetti, F ; Robotti, E ; Marengo, E ; Cecconi, D |
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Zeitschrift: | Analytical biochemistry, Jg. 537 (2017-06-30) |
Veröffentlichung: | 2017 |
Medientyp: | unknown |
ISSN: | 1096-0309 (print) |
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