Impact of anti-PDGFRα antibody surface functionalization on LNC uptake by oligodendrocyte progenitor cells
In: International journal of pharmaceutics, Jg. 618 (2021-10-27), S. 121623
Online
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Zugriff:
Impairment of oligodendrocyte progenitor cell (OPC) differentiation into oligodendrocytes and chronic inflammation are key determinants of poor remyelination observed in diseases such as multiple sclerosis. For many pro-myelinating molecules, the therapeutic potential is hindered by poor solubility or limited access to the targeted cells. A promising approach to improve the delivery of those molecules to OPC is to encapsulate them in functionalized Lipid Nanocapsules (LNC). We aimed to develop the first OPC-targeting LNC, by grafting an anti-PDGFRα antibody on the surface of the LNC using several strategies and evaluating the interaction with PDGFRα via ELISA. We found that only site-selective click-chemistry grafting maintained anti-PDGFRα/PDGFRα association, which was confirmed in vitro on primary rat OPC. In conclusion, we demonstrated that it was possible to produce anti-PDGFRα functionalized LNC, we confirmed the antibody's ability to recognize its receptor after grafting and we optimized techniques to characterize antibody functionalized LNC.
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Impact of anti-PDGFRα antibody surface functionalization on LNC uptake by oligodendrocyte progenitor cells
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Autor/in / Beteiligte Person: | Labrak, Yasmine ; Heurtault, Béatrice ; Frisch, Benoît ; Saulnier, Patrick ; Lepeltier, Elise ; Veronique E Miron ; Muccioli, Giulio G. ; Anne des Rieux ; UCL - SSS/LDRI - Louvain Drug Research Institute ; Louvain Drug Research Institute [Bruxelles, Belgique] (LDRI) ; Université Catholique de Louvain = Catholic University of Louvain (UCL) ; Conception et application de molécules bioactives (CAMB) ; Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) ; Micro et Nanomédecines Translationnelles (MINT) ; Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) ; University of Edinburgh |
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Zeitschrift: | International journal of pharmaceutics, Jg. 618 (2021-10-27), S. 121623 |
Veröffentlichung: | 2021 |
Medientyp: | unknown |
ISSN: | 1873-3476 (print) ; 0378-5173 (print) |
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