Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen
In: Nature Communications, Jg. 11 (2020), Heft 1, S. 1-14
Online
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Zugriff:
Studying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamushi (Ot), an obligate intracellular bacterium that causes the vector-borne human disease scrub typhus. Half the Ot genome is composed of repetitive DNA, and there is minimal collinearity in gene order between strains. Integrating RNA-seq, comparative genomics, proteomics, and machine learning to study the transcriptional architecture of Ot, we find evidence for wide-spread post-transcriptional antisense regulation. Comparing the host response to two clinical isolates, we identify distinct immune response networks for each strain, leading to predictions of relative virulence that are validated in a mouse infection model. Thus, dual RNA-seq can provide insight into the biology and host-pathogen interactions of a poorly characterized and genetically intractable organism such as Ot.
Studying emerging pathogens is often challenging due to the lack of information. Here the authors show that dual RNA-seq, profiling the host and pathogen transcriptome simultaneously, helps uncovering the biology of Orientia tsutsugamushi, a major cause of febrile illness in South-East Asia, and its interaction with the host.
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Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen
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Autor/in / Beteiligte Person: | Giengkam, Suparat ; Mika-Gospodorz, Bozena ; Loo Chien Wang ; Kion-Crosby, Willow ; Wongsantichon, Jantana ; Sobota, Radoslaw M. ; Salje, Jeanne ; Sunyakumthorn, Piyanate ; Vogel, Jörg ; Subbian, Selvakumar ; Barquist, Lars ; Westermann, Alexander J. ; Chuenklin, Suthida ; HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany. |
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Zeitschrift: | Nature Communications, Jg. 11 (2020), Heft 1, S. 1-14 |
Veröffentlichung: | Nature Publishing Group, 2020 |
Medientyp: | unknown |
ISSN: | 2041-1723 (print) |
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