Preservation of sputum samples with cetylpyridinium chloride (CPC) for tuberculosis cultures and Xpert MTB/RIF in a low-income country
BioMed Central, 2017
Online
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Zugriff:
Background Culture contamination with environmental bacteria is a major challenge in tuberculosis (TB) laboratories in hot and humid climate zones. We studied the effect of cetylpyridinium chloride (CPC) preservation on culture results and performance of Xpert MTB/RIF. Methods Consecutive sputum samples from microscopy smear-positive TB patients were collected. Two-hundred samples were equally split in two aliquots, one aliquot was treated with CPC and stored at ambient temperature for 7 days. The second aliquot was immediately processed. Samples were decontaminated for 20, 15 or 10 min, and subsequently cultured on Löwenstein-Jensen medium. Furthermore, 50 samples were stored for 7, 14 and 21 days, and 100 CPC-pretreated samples tested by Xpert MTB/RIF. Results CPC pretreated samples showed a higher culture yield compared to non-treated sputum samples across all decontamination times: 94% vs. 73% at 10 min (p = 0.01), 94% vs. 64% at 15 min (p = 0.004), and 90% vs. 52% at 20 min (p
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Preservation of sputum samples with cetylpyridinium chloride (CPC) for tuberculosis cultures and Xpert MTB/RIF in a low-income country
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Autor/in / Beteiligte Person: | Hella, Jerry ; Sasamalo, Mohamed ; Hiza, Hellen ; Jugheli, Levan ; Mhimbira, Francis ; Doulla, Basra ; Kamwela, Lujeko ; Fenner, Lukas ; Reither, Klaus ; Gagneux, Sebastien |
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Veröffentlichung: | BioMed Central, 2017 |
Medientyp: | unknown |
DOI: | 10.7892/boris.105128 |
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