Soluble c-kit molecule in serum from healthy individuals and patients with haemopoietic disorders
In: British Journal of Haematology, Jg. 91 (1995-09-01), S. 23-29
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Zugriff:
Summary The proto-oncogeae, c-kit, encodes a transmembrane tyrosine kinase receptor (KIT) and plays an important role in haemopoiesis. We have identified a 95kD soluble form of KIT (S-KIT) in culture supernatant of human megakaryoblastic cell line, CMK. To study the physiological significance of S-KIT, we have established a sensitive sandwich ELISA system. Serum samples from healthy individuals contained detectable amounts of S-KIT. Next, we determined a total of 220 samples from 134 patients with haemopoietic disorders. A considerable number of patients with acute myeloid leukaemia (AML), especially those with more immature phenotypes (MO, Ml or M2) had elevated levels of serum S-KIT. Those levels decreased to the normal range after effective chemotherapy. In chronic myeloid leukaemia, patients with myeloid blastic crisis showed markedly elevated levels of serum S-KIT. In contrast, S-KIT levels decreased in cases with either acute or chronic lymphoid leukaemia. There was a tendency for patients with severe aplastic anaemia to show decreased levels, but it was not significant. In myelodysplastic syndrome, S-KIT levels appeared to vary by subsets, with higher concentration in more advanced forms of the disease. Although the functional role of S-KIT is not yet elucidated, these results suggest that the serum S-KIT levels may reflect the pathological states of various haematological disorders.
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Soluble c-kit molecule in serum from healthy individuals and patients with haemopoietic disorders
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Autor/in / Beteiligte Person: | Ohkubo, Toshiya ; Kayoko, Hayakawa ; Miyake, Hirosada ; Kawakita, Makoto ; Yonemura, Ydji ; Hisao, Osawa ; Kitoh, Takashi ; Nakamura, Mitsurd ; Takatsuki, Kiyoshi ; Asou, Norio ; Suda, Toshio |
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Zeitschrift: | British Journal of Haematology, Jg. 91 (1995-09-01), S. 23-29 |
Veröffentlichung: | Wiley, 1995 |
Medientyp: | unknown |
ISSN: | 1365-2141 (print) ; 0007-1048 (print) |
DOI: | 10.1111/j.1365-2141.1995.tb05239.x |
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