Nitric oxide and tumor necrosis factor-α production by Ixeris dentata in mouse peritoneal macrophages
In: Journal of Ethnopharmacology, Jg. 82 (2002-10-01), S. 217-222
Online
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Zugriff:
Using mouse peritoneal macrophages, we have examined the mechanism by which Ixeris dentata (IXD) regulates nitric oxide (NO) production. When IXD was used in combination with recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production. However, IXD had no effect on NO production by itself. The increased production of NO from rIFN-gamma plus IXD-stimulated cells was almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappa B (NF-kappaB). Furthermore, treatment with IXD alone or rIFN-gamma plus IXD in peritoneal macrophages caused a significant increase in tumor necrosis factor-alpha (TNF-alpha) production. PDTC decreased TNF-alpha production induced by IXD significantly. These findings demonstrate that IXD increases the production of NO and TNF-alpha by rIFN-gamma-primed macrophages and suggest that NF-kappaB plays a critical role in mediating these effects of IXD.
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Nitric oxide and tumor necrosis factor-α production by Ixeris dentata in mouse peritoneal macrophages
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Autor/in / Beteiligte Person: | Seong, Kang-Kyung ; Baek, Seung-Hwa ; Jeong, Hyun-Ja ; Chung, Hwan-Suck ; Jeong, Dong-Myong ; Han, Mi-Jung ; Park, Seung-Taeck ; Kim, Hyung-Min ; Myong Jo Kim |
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Zeitschrift: | Journal of Ethnopharmacology, Jg. 82 (2002-10-01), S. 217-222 |
Veröffentlichung: | Elsevier BV, 2002 |
Medientyp: | unknown |
ISSN: | 0378-8741 (print) |
DOI: | 10.1016/s0378-8741(02)00188-5 |
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