The role of insulin–thyroid hormone interaction on β-adrenoceptor-mediated cardiac responses

beta-adrenoceptor-mediated responses are known to be attenuated in diabetic rat hearts, related to decreased receptor sensitivity and density. These impaired responses were improved with insulin in diabetic rats, but not in thyroidectomized diabetic rats. We aimed to investigate the possible interaction between insulin and thyroid hormones to restore diabetes-induced alterations on beta-adrenoceptor-mediated responses. Male Sprague-Dawley rats were divided into seven groups: control (C), diabetic (D), insulin-treated diabetic (DI), thyroidectomized diabetic (TxD), insulin-treated thyroidectomized diabetic (TxDI), insulin+low dose 3,3',5-triiodo-L-thyronine (T-3) treated (TxDIT(2.5)) or insulin+high dose T-3 (TxDIT(5)) treated thyroidectomized diabetic rats. Diabetes was induced with 38 mg/kg streptozotocin. Cardiac function was assessed through pressure-volume analysis and papillary muscle experiments. QPCR and western blot experiments were performed to evaluate cardiac gene expressions. Hemodynamic parameters were impaired in diabetes, and were mostly corrected in DI and TxDIT(5) groups. Isoprenaline- and BRL37344-induced contractile responses were also decreased in diabetes, Isoprenaline responses were improved significantly in DI and TxDIT(5) groups, whereas BRL 37344-mediated responses were increased slightly. Reduced beta(1)-adrenoceptor and SERCA 2A mRNA levels in diabetes were corrected in DI and TxDIT(5) groups. Decreased SERCA 2A and increased beta(3)-adrenoceptor protein levels in diabetes were improved in DI and TxDIT(5) groups. No significant changes were found in phospholamban or endothelial nitricoxide synthase protein levels. These results show that the beneficial effects of insulin on beta-adrenoceptor-mediated responses in diabetic rats are dependent upon adequate concentrations of thyroid hormones. (C) 2013 Elsevier B.V. All rights reserved