CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
In: Molecular cell, Jg. 81 (2021-02-24), Heft 17
Online
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Zugriff:
Summary ATP- and GTP-dependent molecular switches are extensively used to control functions of proteins in a wide range of biological processes. However, CTP switches are rarely reported. Here, we report that a nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly regulated by a CTP switch. In Bacillus subtilis, Noc nucleates on 16 bp NBS sites before associating with neighboring non-specific DNA to form large membrane-associated nucleoprotein complexes to physically occlude assembly of the cell division machinery. By in vitro reconstitution, we show that (1) CTP is required for Noc to form the NBS-dependent nucleoprotein complex, and (2) CTP binding, but not hydrolysis, switches Noc to a membrane-active state. Overall, we suggest that CTP couples membrane-binding activity of Noc to nucleoprotein complex formation to ensure productive recruitment of DNA to the bacterial cell membrane for nucleoid occlusion activity.
Graphical abstract
Highlights • CTP is required for Noc to form a higher-order nucleoprotein complex on DNA • CTP binding switches DNA-entrapped Noc to a membrane-active state • CTP hydrolysis likely reverses the association between Noc-DNA and the membrane • The membrane-targeting helix adopts an autoinhibitory conformation in apo-Noc
Jalal et al. report that the nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly regulated by CTP. CTP binding switches the Noc-DNA complex from a membrane-inactive state to an active state, thus ensuring productive recruitment of DNA to the bacterial cell membrane.
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CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc
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Autor/in / Beteiligte Person: | Ramakrishnan, Karunakaran ; Stevenson, Clare E. M. ; Le, Tung B. K. ; Lawson, David M. ; Errington, Jeff ; Ling Juan Wu ; Tran, Ngat T. ; Rejzek, Martin ; Gobbato, Giulia ; Jalal, Adam S. B. |
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Zeitschrift: | Molecular cell, Jg. 81 (2021-02-24), Heft 17 |
Veröffentlichung: | 2021 |
Medientyp: | unknown |
ISSN: | 1097-4164 (print) |
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