Identification and Characterization of the Multidrug Resistance Gene cfr in a Panton-Valentine Leukocidin-Positive Sequence Type 8 Methicillin-Resistant Staphylococcus aureus IVa (USA300) Isolate
In: Antimicrobial Agents and Chemotherapy, Jg. 54 (2010-12-01), S. 4978-4984
Online
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Zugriff:
The staphylococcal cfr gene mediates resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A, a phenotype that has been termed PhLOPS A . The cfr gene has mainly been associated with coagulase-negative staphylococcal isolates from animals, and only a few cfr -positive methicillin-resistant Staphylococcus aureus (MRSA) isolates have been described so far. This study reports the first description of a cfr -positive MRSA isolate (M05/0060) belonging to the pandemic Panton-Valentine leukocidin (PVL)-positive sequence type 8 MRSA IVa/USA300 (ST8-MRSA-IVa/USA300) clone. The cfr gene was detected in M05/0060 using a DNA microarray which was used to screen PVL-positive MRSA isolates for the presence of virulence genes, typing markers, and antimicrobial resistance genes. Antimicrobial susceptibility testing revealed that M05/0060 exhibited the cfr -associated resistance phenotype. Molecular analysis identified the presence of cfr and a second phenicol resistance gene, fexA , on a novel 45-kb conjugative plasmid, which was designated pSCFS7. Within pSCFS7, a DNA segment consisting of cfr , a truncated copy of insertion sequence IS 21 - 558 , and a region with homology to the DNA invertase gene bin3 of transposon Tn 552 from Bacillus mycoides was integrated into the transposase gene tnpB of the fexA -carrying transposon Tn 558 . The emergence of a multidrug-resistant cfr -positive variant of ST8-MRSA-IVa/USA300 is alarming and requires ongoing surveillance. Moreover, the identification of a novel conjugative plasmid carrying the cfr gene indicates the ability of cfr to spread to other MRSA strains.
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Identification and Characterization of the Multidrug Resistance Gene cfr in a Panton-Valentine Leukocidin-Positive Sequence Type 8 Methicillin-Resistant Staphylococcus aureus IVa (USA300) Isolate
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Autor/in / Beteiligte Person: | Slickers, Peter ; Brennan, Orla M. ; Coleman, David C. ; Monecke, Stefan ; Ehricht, Ralf ; Schwarz, Stefan ; Shore, Anna C. |
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Zeitschrift: | Antimicrobial Agents and Chemotherapy, Jg. 54 (2010-12-01), S. 4978-4984 |
Veröffentlichung: | American Society for Microbiology, 2010 |
Medientyp: | unknown |
ISSN: | 1098-6596 (print) ; 0066-4804 (print) |
DOI: | 10.1128/aac.01113-10 |
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