So far, the development of a unique strategy for specific biomolecules activity monitoring and precise drugs release in cancerous cells is still challenging. Here, we designed a conformation-switchable smart nanoprobe to monitor telomerase activity and to enable activity-triggered drug release in cancerous cells. The straightforward nanoprobe contained a gold nanoparticle (AuNP) core and a dense layer of 5-carboxyfluorescein (FAM)-labeled hairpin DNA shell. The 3′ region of hairpin DNA sequence could function as the telomerase primer to be elongated in the presence of telomerase, resulting in the conformational switch of hairpin DNA. As a result, the FAM fluorescence was activated and the anticancer drug doxorubicin (Dox) molecules which intercalated into the stem region of the hairpin DNA sequence were released into cancerous cells simultaneously. The smart method could specifically distinguish cancerous cells from normal cells based on telomerase activity. It also showed a good performance for monitoring telomerase activity in the cytoplasm by molecular imaging and precise release of Dox triggered by telomerase activity in cancerous cells. These advantages may offer a great potential of this method for monitoring telomerase activity in cancer progression and estimating therapeutic effect.
In dividing cancerous cells, telomerase maintains genome integrity by adding repetitive DNA sequences (TTAGGG) to the chromosome ends. About 90% of tumor entities show telomerase activity in dividing cancerous cells1-3, in contrast, telomerase activity is rarely detected in normal cells4-6. Thus telomerase activity can be considered as an important indicator of cancer progression7,8. And this distinct difference has been a focus for cancer mechanism study and therapeutic strategy development9-11. Thus, the development of a methodology for monitoring cell-to-cell variation in telomerase activity from different samples is urgently needed in biomedical research and clinical practice.
Hitherto, a variety of methods have been developed to investigate the telomerase activity by using various techniques, such as fluorescence12-15, electrochemistry16-19, surface enhanced Raman scattering20, surface plasma resonance21,22, colorimetry23-25, chemiluminescence26, and electrochemiluminescence27,28. However, these technologies can only analyze the bulk samples in vitro, such as cell extraction and urine12,14. They cannot enable us to give insight into telomerase level in complex biological environments, particularly in living cells. Observation of biomolecules in living cells is fundamental to a quantitative understanding of how biological systems function29. So, developing an approach for quantitative and precise detection of telomerase activity in living cells will be of help to understand its function within cancerous cells and its application in the diagnosis of disease.
It has come to light that molecular fluorescence imaging can reveal the activity of biomolecules of interest in living cells30-32, which can be an efficient way to reflect the intracellular activity of telomerase, and also indicate activity change caused by cellular activity33. More recently, several studies are focused on the fluorescence imaging of telomerase based on DNA-functionalized nanoprobes34-38. In these studies, flexible DNA nanostructures are engaged in the detection of telomerase activity. However, some nonnegligible disadvantages remained. The synthesis of these probes needs one or multiple custom-built DNAs to fabricate highly structured nanoprobes. Such structures may not be suitable for practical application, because they may result in unnecessary dissociation of DNA complexes formed by two or more different single-strand DNAs during cellular uptake. Besides, the nanoprobes assembled by heterogeneous DNA hybridization will be challenged by DNA polymerase, causing strand displacement during DNA amplification. These limitations may lead to false positive in complex environment of a cell. To mitigate these limitations, here we simplified the design and construction of the nanoprobes by introducing a rational designed hairpin DNA. The functionalized nanoprobe can be responsive to telomerase activity-triggered DNA conformation switch, while avoiding the interference of polymerase in cells and lowering false positive in complex environment of a cell.
In our design, the nanoprobe is composed of a gold nanoparticle (AuNP) core and a shell with a dense layer of hairpin DNAs. These hairpin DNAs are covalently linked to gold nanoparticles (AuNPs) through Au-S bonds. The 3′ region of hairpin DNA sequence is designed as a primer that could be elongated by telomerase, and the detailed sequences are summarized in Table S1. The fluorophore FAM modified in the stem part of hairpin DNA is quenched as a result of proximity to AuNP surface. In the presence of telomerase, the primer is elongated to produce telomeric repeats (TTAGGG), which trigger the competitive hybridization with the stem part of hairpin DNA. As a result, the 5′ region of hairpin DNA sequence is substituted by the extension chain, giving rise to subsequent DNA conformational switch. The DNA conformational switch produces new hairpin DNA to make the fluorescence of FAM recovered. To improve the performance of the nanoprobe, a four-base pairs of DNA is designed into the 3′ terminal region of hairpin DNA sequence, which could facilitate the DNA conformational change in the presence of telomerase. Therefore, our proposed method can reveal the telomerase activity in the cytoplasm through the conformational switch of hairpin DNA in cancerous cells. On the basis of the obvious difference in telomerase activity between cancerous cells and normal cells, telomerase activity-responsive drug carriers will facilitate more precise and easily controlled drug release than the conventional stimuli-driven drug release system34,36,39. Thus, we have further used the nanoprobe to load Dox molecules via GC base pairs-Dox interaction40 and the telomerase activity-triggered conformation switch has been observed to be able to induce specific release of trapped Dox molecules into cancerous cells.
In this work, we proposed a straightforward method by using a conformation-switchable smart nanoprobe for telomerase imaging in cancerous cells and telomerase activity-responsive Dox release. Its architecture and design mechanism were illustrated in Fig. 1. In detail, the nanoprobe was composed of an AuNP core and a dense layer of hairpin DNAs (HP-1) (Fig. 1A). The HP-1 was conjugated on AuNPs via Au-S bonds at 5′ end of the sequence. Due to the stem-loop structure of HP-1, FAM modified in the stem of HP-1 could be close to the surface of AuNPs, resulting in the fluorescence quenching. Dox molecules were inserted into the GC base pairs of the stem part, their fluorescence were quenched because of GC base pairs-Dox interaction. As illustrated in Fig. 1B,C, in the presence of telomerase, the free 3′ region of HP-1, which was not hybridized with 5′ region of HP-1, would function as the primer of telomerase to be elongated to generate telomeric repeats. The elongated telomeric repeats could competitively hybridize with the stem part of HP-1. As a result, the 5′ region of HP-1 was substituted by telomeric repeats and the loop was opened subsequently. This substitution process triggered the conformational switch of HP-1 to form a new hairpin DNA. As the most direct effect, the quenched FAM was lighted up and the trapped Dox molecules were released into cancerous cells for specific telomerase activity monitoring and precise Dox release.Schematic illustration of telomerase activity monitoring and drug release based on DNA conformational switch triggered by telomerase activity in living cells. (A) The synthesis procedure of AuNP-MPs. (B) The detailed conformational switch process. (C) The intracellular imaging and drug release based on DNA conformational switch initiated by telomerase activity.
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To enhance the stability and reduce the immunogenicity of Dox-loaded AuNP-MPs (Dox-AuNP-MPs), thiolated polyethylene glycol (PEG-SH) was modified on AuNPs. The performance of the AuNP-MPs was improved by linking a four-base pairs of DNA with the 3′ terminal region of HP-1, as shown in Fig. 1B. A four-base pairs of DNA was expected to form an intramolecular toehold for facilitating the DNA conformational change after the primer elongation. Specifically, the intramolecular toehold was a short DNA sequence (ACTG), which could form an unstable small hairpin DNA in the absence of telomerase. Upon addition of telomerase, the toehold sequence could first act as the primer to be elongated by telomerase, and then the formation of small hairpin DNA produced an intramolecular toehold to further enhance the competitive hybridization of the elongated telomeric repeats with the stem part of the HP-1.
The AuNP-MPs for telomerase activity monitoring were successfully synthesized and characterized (Fig. S1). After AuNPs were functionalized with HP-1, the typical absorption peak of AuNPs in the UV-vis absorption spectra was red-shifted from 520 nm to 523 nm (curve a, b, Fig. S1), and the characteristic peak of DNA at 260 nm appeared compared to unmodified AuNPs. Furthermore, the increased absorption value at 260 nm was observed after telomerase elongation compared to that of AuNP-MPs in the absence of telomerase (curve c, Fig. S1). The average diameter of AuNP-MPs characterized via TEM was approximately 13 nm, which was similar to that of bare AuNPs (Fig. S2A,B). But the modification of PEG made the AuNP-MPs with higher dispersibility than bare AuNPs, because PEG as an electrically neutral ligand effectively restrained the aggregation of AuNP41-43. As illustrated in Fig. S1C,D, dynamic light scattering data showed that the hydrodynamic diameter of AuNP-MPs was 38.3 nm, which of AuNPs was 21.9 nm, indicating that the surface load increased the diameter of nanoprobe. These results suggested that the hairpin DNA were successfully linked on the surface of AuNPs. In addition, the amount of hairpin DNA loaded on each AuNP was estimated to be about 27 (Fig. S3).
Once we validated that the hairpin DNA was successfully conjugated on AuNPs, we sought to test whether the prepared AuNP-MPs could make expectative response to telomerase activity. But before that, telomeric repeat amplification protocol (TRAP) assay was performed to validate the feasibility of HP-1 as primer of telomerase, as a positive control, TS primer was also used for telomerase substrate. As a result, the 6-bp incremental ladder produced by various concentrations of telomerase could be visualized in gel (Fig. S4A). In order to further verify that these incremental ladders were indeed associated with telomerase activity, TRAP assay was performed by incubating with the epigallocatechin gallate (EGCG)-treated cell extracts, since EGCG is a well-characterized telomerase inhibitor. The results showed that the incremental ladder and the brightness of bands decreased with the increasing concentration of EGCG (Fig. S4B). So, the results showed that HP-1 could be indeed used as telomerase substrate, and the telomerase activity could be inhibited by EGCG. Next, the response of AuNP-MPs to telomerase activity was tested, as shown in Fig. 2. Bare AuNPs had no observable fluorescence signal in 1× TRAP reaction buffer (curve a, Fig. 2). The AuNP-MPs were incubated with dNTPs in 1× TRAP reaction buffer at 37 °C in the absence of telomerase, which exhibited no obvious fluorescent signal, suggesting that the FAM fluorescence was effectively quenched by the AuNPs (curve b, Fig. 2). Upon the addition of telomerase extraction, the significant enhancement in fluorescence intensity was observed (curve d, Fig. 2). The results confirmed that telomerase activity-induced fluorescence switch based on intramolecular conformational switch of HP-1 was successfully constructed. In our work, we introduced a four-base pairs of toehold to enhance the response of AuNP-MPs to telomerase activity. Next, to verify the actual effect of the design, four bases were deleted to form a shortened hairpin DNA (HP-2) which was lack of toehold. HP-2-modified AuNPs (AuNP-MPs2) were incubated with the telomerase extraction, and then 40% decrease in the fluorescence intensity than that of AuNP-MPs with the intramolecular toehold was observed (curve c, Fig. 2). The result suggested that the introduction of toehold improved the release of FAM from the AuNPs surface, in other words, more hairpin DNA sequences performed the DNA chain exchanges with the help of toehold. On the contrary, as a control, HP-3 was designed to construct AuNP-MPs3, which could not be triggered to induce conformational change due to lack of complementary sequence of telomeric repeats (TTAGGG) and intramolecular toehold (ACTG) (curve e, Fig. 2). In view of above results, the HP-1 containing an intramolecular toehold was used in the subsequent experiments.Fluorescence response of bare AuNPs (a), HP-1 (with toehold)-modified AuNPs (AuNP-MPs) in the absence of telomerase (b), HP-2 (without toehold)-modified AuNPs (AuNP-MPs2) after incubation with telomerase for 2 h (c), HP-1 (with toehold)-modified AuNPs (AuNP-MPs) after incubation with telomerase for 2 h (d) and HP-3-modified AuNPs (AuNP-MPs3) after incubation with telomerase for 2 h (e).
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To obtain the optimal detection performance, the incubation time was investigated. As illustrated in Fig. S5, a gradual increase of the fluorescence intensity of AuNP-MPs with an increasing incubation time was observed, and the fluorescence intensity plateaued after 2 h. Thus, the incubation time for telomerase elongation reaction was chosen to be 2 h.
The next experiments were performed to test the stability of the prepared AuNP-MPs against damage by intracellular enzymes and reductants. DNase I, T4 DNA ligase, GSH, DNA polymerase I and DTT were individually incubated with AuNP-MPs. Both GSH and T4 DNA ligase had almost no effect on the fluorescent signal (Fig. S6A). More remarkable, the results also manifested that the HP-1 conjugated on the surface of AuNPs were not degraded by DNase I. But, by contrast, on addition of the telomerase extraction or the strong reductant DTT, obvious fluorescence recovery was detected. Unlike DNA conformational change caused by telomerase elongation, the fluorescence recovery resulted from DTT treatment was attributed to the break of the Au-S bond and detachment of DNA from AuNPs surface44. To test the performance of the synthesized nanoprobe against DNA polymerase, DNA polymerase I (Klenow fragment) was chosen as a model. After incubation with DNA polymerase I for 2 h in the presence of dNTPs, the fluorescence intensity of AuNP-MPs had little increase than that of AuNP-MPs without dNTPs, which may be caused by DNA polymerase I elongation (Fig. S6B). After 12 h, the fluorescence intensity of AuNP-MPs increased further regardless of the dNTPs change. The results suggested that the fluorescence increase after 12 h were not due to the strand displacement caused by DNA polymerase elongation, it was speculated to be caused mainly by the DTT in the DNA polymerase. The DTT was added to protect DNA polymerase by the manufacturer. Therefore, according to the above results, the fluorescence intensity increase caused by DNA polymerase I could be ignored during telomerase activity monitoring. The stability of AuNP-MPs was also confirmed in different media, such as 10% FBS, DMEM with 10% FBS, PBS and whole blood (Fig. S7). In summary, these results suggested that the telomerase-specificity and structural stability of AuNP-MPs, showed the favorable for telomerase detection and cellular applications.
The validity of the designed AuNP-MPs for telomerase activity analysis was evaluated in following work. In optimized condition, we measured the fluorescence intensity changes in response to the telomerase extracts from various concentrations of HeLa cells. As expected, higher concentration of the telomerase extract led to larger fluorescence increase, which wonderfully verified our design. A linear relationship was observed between the fluorescence intensity and telomerase extracts from 0 to 4000 cells/mL, as demonstrated in Fig. 3. The regression equation was calculated to be y = 511.25 × +0.547, the correlation coefficient (R
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It is of great importance for cancer early diagnosis and therapy through evaluating the telomerase activity in living cells. Therefore, based on the results above, we further utilized this AuNP-MPs to monitor the telomerase activity in several different cancer cells, including HeLa (human cervical cancer cell line), MCF-7 (human breast cancer cell line), A549 (human lung adeno carcinoma epithelial cell line), MDA-MB-231 (human breast cancer cell line), and L-02 (normal human hepatocyte cell line). First of all, to obtain the optimal imaging results, the AuNP-MPs were incubated with HeLa cells for different time periods. The confocal images revealed that the fluorescence intensity inside the cells increased with the prolonged incubation time (Fig. S8). After incubating with HeLa cells for 2 h, the fluorescent signal reached the maximum. For this reason, 2 h of incubation time was further used for cellular imaging in the subsequent experiments. Importantly, these images revealed that the prepared AuNP-MPs could be efficiently internalized and sensitively responded by HeLa cells. The verified results are the prerequisite for telomerase detection and drug delivery in cancerous cells. Subsequently, the confocal images of the AuNP-MPs internalized into MCF-7, A549, MDA-MB-231, and L-02 cells were acquired via the confocal microscopy. As indicated in Fig. 4, all cancer cell lines demonstrated distinct telomerase activity, but no telomerase activity was found in the L-02 cells. The distinguishing fluorescence intensity between cancerous and noncancerous cells signified that the AuNP-MPs were successfully lighted up because of the DNA conformational switch induced by telomerase activity in cancerous cells. Due to the nuclear membrane barrier function, our designed nanoprobes can hardly be internalized into nucleus without a nuclear localization signal, especially, in a short time. So the green fluorescence was observed predominantly and even exclusively in the cytoplasm of HeLa cells (Fig. 4). The flow cytometric analysis was performed to detect the fluorescence signal of the AuNP-MPs in different cells, which was similar to the confocal images (Fig. S9A). To exclude the possibility that differences in fluorescence signal was caused by AuNP-MPs uptake, the inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to quantify the relative amount of AuNP-MPs in each cell, as shown in Fig. S10A. As a result, HeLa, MCF-7 and L02 cells showed similar uptake of AuNP-MPs after incubation for 2 h. So, the differences in fluorescence intensity between cancer cells and L-02 cells were associated with telomerase activity rather than the total amount of AuNP-MPs inside the cells. Therefore, the AuNP-MPs exhibit a promising potential for monitoring telomerase activity in living cells and distinguishing cancerous cells from normal cells.Confocal images for evaluation of DNA conformational switch-based AuNP-MPs for telomerase activity monitoring in individual cells: HeLa, MCF-7, A549, MDA-MB-231, and L-02 cells.
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To confirm that the fluorescence recovery in cells was indeed due to telomerase activity, we incubated HeLa cells with various levels of EGCG. The results were demonstrated in Fig. 5, which showed the fluorescence intensity gradually reduced with increasing concentrations of EGCG. The conclusion was also proved via TRAP assay (Fig. S4B) and flow cytometry (Fig. S8B). These results confirmed that the AuNP-MPs were specific to telomerase activity, which could be used to precisely reflect telomerase activity in cancerous cells.Confocal images of HeLa cells after incubation with different concentrations of EGCG (0 μM, 10 μM, 20 μM, or 40 μM) for 24 h, and then incubated with 100 μL 1.2 nM AuNP-MPs for 2 h.
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Considering the efficient cellular uptake of AuNP-MPs, we further constructed a telomerase activity-initiated drug release nanocarrier by intercalating Dox molecules into the GC base pairs of the stem of HP-1. The fluorescence of Dox could be quenched after inserting into HP-1. Upon hairpin structure was unfolded because of DNA conformational switch caused by telomerase elongation, the Dox molecules could be released into cancerous cells. It was calculated that approximately 154 Dox molecules could be loaded on one AuNP-MP (Fig. S11). The design was verified through in vitro experiments (Fig. S12), the fluorescence intensity of the released Dox from the hairpin DNA was enhanced with the increasing reaction time of telomerase elongation. The recovered fluorescence of Dox was consistent with that of FAM. Furthermore, as shown in Fig. S13, higher concentration of the telomerase extract led to higher Dox fluorescence increase, indicating more Dox release from Dox-AuNP-MPs, and reached equilibrium at 1 × 10
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The next topic of interest was the tumor inhibition efficacy of the Dox-AuNP-MPs. To examine the tumor inhibition efficacy, MTT assay was conducted on HeLa and L-02 cells (Fig. S16). No obvious decrease of cell viability was found in L-02 cells in AuNPs, AuNP-MPs, or Dox-AuNP-MPs-treated groups, compared to the free Dox group. By contrast, in HeLa cells, Dox or Dox-AuNP-MPs treatment resulted in a significant reduction in cell survival, which was due to telomerase-specific Dox release into HeLa cells from Dox-AuNP-MPs. From another aspect, the above results also suggested that the cytotoxicity of AuNPs and AuNP-MPs on cells were very little. Therefore, the cell MTT assay demonstrated the specific and precise cancerous cell inhibition of Dox-AuNP-MPs.
In this study, we have successfully synthesized a smart nanoprobe, which could be responsive to telomerase activity-triggered intramolecular DNA conformation switch specifically. The nanoprobe could be simply designed and constructed. The nanoprobe could also be used for the detection of telomerase activity of HeLa cell extracts in vitro, the LOD of which was comparable to the previous reports (Table S2). By taking advantages of the significant merits of the AuNP-MPs, we have successfully used the AuNP-MPs to monitor the telomerase activity in vivo with cancerous cells-specific targeting. This smart nanoprobe is functionalized with the intramolecular DNA conformation switch structure, which could be avoid effectively the interference of polymerase in cells, lower false positive in complex environment, and showed a good stability in different medium. As a drug nanocarrier, it efficiently releases drug to cancerous cells that is caused by telomerase activity. Given these advantages, the straightforward strategy based on AuNP-MPs shows a promising potential for aiding in specific cancerous cells recognition and reducing the undesired death of healthy cells that is commonly confronted in conventional chemotherapy.
Chloroauric acid tetrahydrate (HAuCl
AuNPs with an average diameter of 13 nm were prepared by citrate reduction method45,46. Briefly, 4 mL sodium citrate solution (19.4 mM) was added rapidly into a boiling 40 mL HAuCl
To prepare Dox-AuNP-MPs, 20 µM Dox was incubated with the AuNP-MPs in Tris-HCl and shaken overnight. The mixture was centrifuged at 14000 rpm for 10 min and washed twice with Tris-HCl, then re-dispersed in Tris-HCl for the next experiments.
The morphologies of the synthesized AuNPs and AuNP-MPs were acquired with an H-7650 transmission electron microscopic (Hitachi, Japan). The particle sizes of AuNPs and AuNP-MPs were characterized by a Zetaplus/90plus dynamic light scattering instrument (Brookhaven Instrument Co., USA). The ultraviolet-visible (UV-vis) spectra were recorded using a Shimadzu UV-1800 spectrometer (Shimadzu Inc., Kyoto, Japan).
The AuNP-MPs were prepared according to the above synthesis procedures. Then 200 μL AuNP-MPs (2 nM) were treated with 20 mM DTT and incubated together for 12 h under shaking at room temperature. The mixture was centrifuged at 14000 rpm for 10 min, and the supernatant was collected for fluorescence measurement with a Hitachi F-7000 fluorescence spectrophotometer at the emission wavelength of 520 nm. To quantitate the number of hairpin DNA on one AuNP, the fluorescence of a dilution series of hairpin DNA with known concentrations was detected to generate standard curve.
20 µM Dox was incubated with prepared AuNP-MPs and shaken overnight. The mixture was separated to obtain the supernatant, subsequently its fluorescence intensity was measured with a fluorescence spectrometer. Under the same condition, a standard linear calibration curve of Dox was generated. Finally, the molar concentration of Dox could be converted by the fluorescence intensity.
HeLa, MCF-7, MDA-MB-231, and A549 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Gibco), penicillin (100 µg/mL), and streptomycin (100 µg/mL). L-02 cells were cultivated in Roswell Park Memorial Institute 1640 medium (RPMI 1640, Gibco) supplemented with 10% FBS, penicillin (100 µg/mL), and streptomycin (100 µg/mL). All cells were maintained at 37 °C in a humidified atmosphere with 5% CO
To extract telomerase, cells were collected during the exponential phase of growth. Cells were dispersed in a 1.5-mL EP tube and rinsed twice with ice-cold PBS solution (10 mM Na
0.5 μM HP-1 or TS primer was first extended by incubating with different concentration of telomerase extracts in the presence of 250 μM dNTPs at 37 °C. After 2 h, the extension products were amplified by PCR in the presence of CX primer: 95 °C for 5 min to inactivate telomerase, and 40 cycles at 95 °C for 30 s, 52 °C for 30 s and 72 °C for 30 s. Following PCR, 2 μL GelRed dye was added to each TRAP reaction mixture and run on a 12% nondenaturing acrylamide gel in 1× TBE. To validate EGCG-induced telomerase activity inhibition, HP-1 was incubated with different concentration of EGCG-treated telomerase extract, and then PCR experiment was performed with same conditions.
Telomerase activity detection of HeLa cell extract was performed by mixing variable amounts of cell extract with 4 nM AuNP-MPs in the presence of 400 μM dNTPs. After incubating at 37 °C for 2 h, the fluorescence intensity of the mixture was detected under excitation at 493 nm.
20 mM DTT, 0.4 mg/mL DNase I, 4 U/mL T4 DNA ligase, and 15 mM GSH were mixed with AuNP-MPs (4 nM), respectively. After incubation for 12 h in 200 μL 1× TRAP reaction buffer containing 20 mM Tris-HCl (pH 8.3), 1.5 mM MgCl
DNA polymerase I (Klenow fragment) was chosen as a model to test the stability of AuNP-MPs against DNA polymerase. 4 U/mL DNA polymerase I was incubated with 4 nM AuNP-MPs at 37 °C in Tirs-HCl (10 mM Tris, 10 mM MgCl
Dox-AuNP-MPs (4 nM) were mixed with 400 μM dNTPs in 1× TRAP reaction buffer and incubated at 37 °C for 2 h after addition of different concentration of telomerase extraction to obtain the fluorescence intensity of Dox.
HeLa, MCF-7, A549, MDA-MB-231, and L-02 cells (1 mL, 1 × 10
After HeLa cells (1 mL, 1 × 10
HeLa cells were seeded into the confocal dishes and cultured at 37 °C for 24 h with 5% CO
HeLa, MCF-7, and L-02 cells were seeded into the individual culture dish for 24 h, respectively. 100 μL Dox-AuNP-MPs were added into each dish and incubated for 2 h. Cells were then washed twice with ice-cold PBS and imaged with confocal microscopy.
Different concentrations of EGCG (0 μM, 10 μM, 20 μM, or 40 μM) were incubated with HeLa cells for 24 h in confocal dish before detecting telomerase activity-triggered Dox release from Dox-AuNP-MPs with fluorescent confocal imaging.
ICP-OES measurements were conducted on an Agilent 730 ICP-OES. Operating conditions of the ICP-OES are listed below: RF power: 1000 W; plasma Ar flow rate: 15 L/min; auxiliary gas flow rate: 1.5 L/min; nebulizer pressure: 200 kPa. AuNP-MPs and Dox-AuNP-MPs (4 nM) was incubated with HeLa, MCF-7 and L-02 cells in 6 well plates. After 2 h, all cells were washed three times with PBS buffer and then lysis buffer (200 µL) was added to the cells. The resulting cell lysate was digested overnight using 3 mL of HNO
HeLa, MCF-7, A549, and L-02 cells were seeded into a 6-well plate at a concentration of 1 × 10
Various concentrations of EGCG (0 μM, 10 μM, 20 μM, or 40 μM) were added into the 6-well plate and incubated with HeLa cells for 24 h. 100 μL AuNP-MPs (12 nM) were added into the 6-well plate and incubated at 37 °C for 2 h. The culture medium was removed and the cells were washed twice by using ice-cold PBS. Finally, the cells were trypsinized and collected for flow cytometry to detect fluorescence intensity.
HeLa and L-02 cells were seeded and cultured for 24 h in 96-well plates at a concentration of 1 × 10
PHOTO (COLOR): Supplementary Information
This work was supported by the National Natural Science Foundation of China (Grant Nos 81772593, 81672570), and the China Postdoctoral Science Foundation (Grant. 2017M611532).
H.S. and T.G. performed the major experiments, L.S., T.C. and Y.L. prepared the cell samples, H.S., Y.X. and G.L. wrote the manuscript.
The authors declare no competing interests.
Supplementary information accompanies this paper at 10.1038/s41598-018-34670-7.
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By Hai Shi; Tao Gao; Liu Shi; Tianshu Chen; Yang Xiang; Yuanyang Li and Genxi Li