Direct analysis of Stevia leaves for diterpene glycosides by desorption electrospray ionization mass spectrometry
In: The Analyst, Jg. 134 (2009), S. 867-867
Online
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Zugriff:
The analysis of Stevia leaves has been demonstrated without any sample preparation using desorption electrospray ionization (DESI) mass spectrometry. Direct rapid analysis was achieved using minimal amounts of sample ( approximately 0.15 cm x 0.15 cm leaf fragment). Characteristic constituents of the Stevia plant are observed in both the positive and negative ion modes including a series of diterpene 'sweet' glycosides. The presence of the glycosides was confirmed via tandem mass spectrometry analysis using collision-induced dissociation and further supported by exact mass measurements using an LTQ-Orbitrap. The analysis of both untreated and hexane-extracted dry leaves proved that DESI can be successfully used to analyze untreated leaf fragments as identical profiles were obtained from both types of samples. Characterization and semi-quantitative determination of the glycosides was achieved based on the glycoside profile within the full mass spectrum. In addition, the presence of characteristic glycosides in an all-natural commercial Stevia dietary supplement was confirmed. This study provides an example of the application of DESI to direct screening of plant materials, in this case diterpene glycosides.
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Direct analysis of Stevia leaves for diterpene glycosides by desorption electrospray ionization mass spectrometry
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Autor/in / Beteiligte Person: | Tata, Alessandra ; Perry, Richard H. ; Haas George, W ; Jackson, Ayanna U. ; West, Leslie G. ; Wu, Chunping ; R. Graham Cooks |
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Zeitschrift: | The Analyst, Jg. 134 (2009), S. 867-867 |
Veröffentlichung: | Royal Society of Chemistry (RSC), 2009 |
Medientyp: | unknown |
ISSN: | 1364-5528 (print) ; 0003-2654 (print) |
DOI: | 10.1039/b823511b |
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