OSR1 and SPAK cooperatively modulate Sertoli cell support of mouse spermatogenesis
In: Scientific Reports, Jg. 6 (2016-11-17)
Online
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Zugriff:
We investigated the role of oxidative stress-responsive kinase-1 (OSR1) and STE20 (sterile 20)/SPS1-related proline/alanine-rich kinase (SPAK), upstream regulators of the Na+-K+-2Cl− cotransporter (NKCC1)—essential for spermatogenesis—in mouse models of male fertility. Global OSR1+/− gene mutations, but not global SPAK−/− or Sertoli cell (SC)-specific OSR1 gene knockout (SC-OSR1−/−), cause subfertility with impaired sperm function and are associated with reduced abundance of phosphorylated (p)-NKCC1 but increased p-SPAK expression in testicular tissue and spermatozoa. To dissect further in a SC-specific manner the compensatory effect of OSR1 and SPAK in male fertility, we generated SC-OSR1−/− and SPAK−/− double knockout (DKO) male mice. These are infertile with defective spermatogenesis, presenting a SC-only-like syndrome. Disrupted meiotic progression and increased germ cell apoptosis occurred in the first wave of spermatogenesis. The abundance of total and p-NKCC1 was significantly decreased in the testicular tissues of DKO mice. These results indicate that OSR1 and SPAK cooperatively regulate NKCC1-dependent spermatogenesis in a SC-restricted manner.
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OSR1 and SPAK cooperatively modulate Sertoli cell support of mouse spermatogenesis
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Autor/in / Beteiligte Person: | Chen, Shyi-Jou ; Sytwu, Huey-Kang ; Yang, Sung-Sen ; Huang, Hsin-Hui ; Wu, Gwo-Jang ; Yu, Mu-Hsien ; Lin, Shih-Hua ; Chu, Chin-Chen ; Lin, Yu-Chun ; Chang, Fung-Wei ; Liu, Yung-Liang |
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Zeitschrift: | Scientific Reports, Jg. 6 (2016-11-17) |
Veröffentlichung: | Springer Science and Business Media LLC, 2016 |
Medientyp: | unknown |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/srep37205 |
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