Vagotomy ameliorates islet morphofunction and body metabolic homeostasis in MSG-obese rats
In: Brazilian Journal of Medical and Biological Research v.48 n.5 2015 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 48, Issue: 5, Pages: 447-457, Published: 24 FEB, Jg. 48 (2015), Heft 5, S. 447-457
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Zugriff:
The parasympathetic nervous system is important for β-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic β-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline [control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca(2+) mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and β-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and β-cell area/pancreas section, respectively. Also, the β-cell number per β-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca(2+) mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater β-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats.
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Vagotomy ameliorates islet morphofunction and body metabolic homeostasis in MSG-obese rats
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Autor/in / Beteiligte Person: | Maria Lúcia Bonfleur ; Jean Franciesco Vettorazzi ; Rosane Aparecida Ribeiro ; Lubaczeuski, Camila ; Junia Carolina Santos-Silva ; Sandra Lucinei Balbo ; Carneiro, Everardo M. |
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Zeitschrift: | Brazilian Journal of Medical and Biological Research v.48 n.5 2015 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 48, Issue: 5, Pages: 447-457, Published: 24 FEB, Jg. 48 (2015), Heft 5, S. 447-457 |
Veröffentlichung: | Associação Brasileira de Divulgação Científica, 2015 |
Medientyp: | unknown |
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