Regulation of expression of the uxu operon and of the uxuR regulatory gene in Escherichia coli K12
In: Journal of general microbiology, Jg. 129 (1983-11-01), Heft 11
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Zugriff:
Gene fusions between the lac structural genes and various genes of the hexuronate system of Escherichia coli K12 were isolated by the technique of Casadaban. Mud(Aprlac) and lambda plac-Mu insertion mutants were constructed in which the lac genes were fused to the regulatory region of the uxu operon. In all the uxu-lac fusion strains, beta-galactosidase expression was shown to be inducible by the natural inducers of the uxu operon (glucuronate and fructuronate) and sensitive to catabolite repression by glucose. In addition we isolated a Mud(Aprlac) fusion where the lac genes were fused to the uxuR regulatory gene. In this fusion the synthesis of beta-galactosidase reflects the regulation of the uxuR gene. In the presence of a wild-type uxuR allele, partial repression of beta-galactosidase expression was found; the repression was removed when inducer was added. This result indicates that while the uxuR gene is subject to autogenous control, the uxuR repressor may have only a low affinity for its own operator.
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Regulation of expression of the uxu operon and of the uxuR regulatory gene in Escherichia coli K12
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Autor/in / Beteiligte Person: | Robert-Baudouy, Janine ; Hugouvieux-Cotte-Pattat, Nicole |
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Zeitschrift: | Journal of general microbiology, Jg. 129 (1983-11-01), Heft 11 |
Veröffentlichung: | 1983 |
Medientyp: | unknown |
ISSN: | 0022-1287 (print) |
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