Proteomic Analysis of Antigen 60 Complex of M. bovis Bacillus Calmette-Guérin Reveals Presence of Extracellular Vesicle Proteins and Predicted Functional Interactions

Mary L Garcia ; Paul R Sanders ; et al.

In: Vaccines, Jg. 7 (2019-08-01), Heft 3

Online unknown

Zugriff:

Tuberculosis (TB) is ranked among the top 10 causes of death worldwide. New biomarker-based serodiagnostics and vaccines are unmet needs stalling disease control. Antigen 60 (A60) is a thermostable mycobacterial complex typically purified from Bacillus Calmette-Gué

rin (BCG) vaccine. A60 was historically evaluated for TB serodiagnostic and vaccine potential with variable findings. Despite containing immunogenic proteins, A60 has yet to be proteomically characterized. Here, commercial A60 was (1) trypsin-digested in-solution, analyzed by LC-MS/MS, searched against M. tuberculosis H37Rv and M. bovis BCG Uniprot databases

(2) analyzed using STRING to predict protein&ndash

protein interactions

and (3) probed with anti-TB monoclonal antibodies and patient immunoglobulin G (IgG) on Western blot to evaluate antigenicity. We detected 778 proteins in two A60 samples (440 proteins shared), including DnaK, LprG, LpqH, and GroEL1/2, reportedly present in mycobacterial extracellular vesicles (EV). Of these, 107 were also reported in EVs of M. tuberculosis, and 27 key proteins had significant protein&ndash

protein interaction, with clustering for chaperonins, ribosomal proteins, and proteins for ligand transport (LpqH and LprG). On Western blot, 7/8 TB and 1/8 non-TB sera samples had reactivity against 37&ndash

50 kDa proteins, while LpqH, GroEL2, and PstS1 were strongly detected. In conclusion, A60 comprises numerous proteins, including EV proteins, with predicted biological interactions, which may have implications on biomarker and vaccine development.

Titel:	Proteomic Analysis of Antigen 60 Complex of M. bovis Bacillus Calmette-Guérin Reveals Presence of Extracellular Vesicle Proteins and Predicted Functional Interactions
Autor/in / Beteiligte Person:	Mary L Garcia ; Paul R Sanders ; Arifin, Norsyahida ; Othman, Nurulhasanah ; Anderson, David A. ; Khayriyyah Mohd Hanafiah
Link:	View record in OpenAIRE (Volltext) https://www.mdpi.com/2076-393X/7/3/80
Zeitschrift:	Vaccines, Jg. 7 (2019-08-01), Heft 3
Veröffentlichung:	MDPI AG, 2019
Medientyp:	unknown
Schlagwort:	0301 basic medicine Antigenicity medicine.drug_class 030106 microbiology Immunology lcsh:Medicine Biology Monoclonal antibody Article Immunoglobulin G Chaperonin Microbiology 03 medical and health sciences Antigen Western blot Ribosomal protein Drug Discovery medicine Pharmacology (medical) BCG mass spectrometry Pharmacology medicine.diagnostic_test lcsh:R biomarkers Extracellular vesicle 030104 developmental biology Infectious Diseases protein–protein interaction tuberculosis biology.protein extracellular vesicles
Sonstiges:	Nachgewiesen in: OpenAIRE Sprachen: English File Description: application/pdf Language: English Rights: OPEN

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.