Amorphous Surface Layer versus Transient Amorphous Precursor Phase in Bone - A Case Study Investigated by Solid-state NMR Spectroscopy
In: Acta Biomaterialia Acta Biomaterialia, 2017, 59, pp.351-360. ⟨10.1016/j.actbio.2017.06.040⟩ Acta Biomaterialia, Elsevier, 2017, 59, pp.351-360. ⟨10.1016/j.actbio.2017.06.040⟩; (2017)
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Zugriff:
International audience; The presence of an amorphous surface layer that coats a crystalline core has been evidenced for many biominerals, including bone mineral. In parallel, transient amorphous precursor phases have been proposed in various biomineralization processes, including bone biomineralization. Here we propose a methodology to investigate the origin of these amorphous environments taking the bone tissue as a key example. This study relies on the investigation of a bone tissue sample and its comparison with synthetic calcium phosphate samples, including a stoichiometric apatite, an amorphous calcium phosphate sample, and two different biomimetic apatites.To reveal if the amorphous environments in bone originate from an amorphous surface layer or a transient amorphous precursor phase, a combined solid-state nuclear magnetic resonance (NMR) experiment has been used. The latter consists of a double cross polarization 1H→31P→1H pulse sequence followed by a 1H magnetization exchange pulse sequence. The presence of an amorphous surface layer has been investigated through the study of the biomimetic apatites; while the presence of a transient amorphous precursor phase in the form of amorphous calcium phosphate particles has been mimicked with the help of a physical mixture of stoichiometric apatite and amorphous calcium phosphate. The NMR results show that the amorphous and the crystalline environments detected in our bone tissue sample belong to the same particle. The presence of an amorphous surface layer that coats the apatitic core of bone apatite particles has been unambiguously confirmed, and it is certain that this amorphous surface layer has strong implication on bone tissue biogenesis and regeneration.
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Amorphous Surface Layer versus Transient Amorphous Precursor Phase in Bone - A Case Study Investigated by Solid-state NMR Spectroscopy
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Autor/in / Beteiligte Person: | Nassif, Nadine ; Chan-Chang, Tsou-Hsi-Camille ; Ajili, Widad ; Delices, Annette ; Azaïs, Thierry ; Laurent, Guillaume ; Babonneau, Florence ; Stanislas Von Euw ; Spectroscopie, Modélisation, Interfaces pour L'Environnement et la Santé (SMiLES) ; Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) ; Matériaux Hybrides et Nanomatériaux (MHN) ; Matériaux Hybrides et Procédés (LCMCP-MHP ) ; Matériaux Hybrides et Nanomatériaux (LCMCP-MHN) ; Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP) ; Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC) |
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Quelle: | Acta Biomaterialia Acta Biomaterialia, 2017, 59, pp.351-360. ⟨10.1016/j.actbio.2017.06.040⟩ Acta Biomaterialia, Elsevier, 2017, 59, pp.351-360. ⟨10.1016/j.actbio.2017.06.040⟩; (2017) |
Veröffentlichung: | HAL CCSD, 2017 |
Medientyp: | unknown |
ISSN: | 1742-7061 (print) |
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