Mesoderm Induction in Amphibians: the Role of TGF-β2-Like Factors
In: Science, Jg. 239 (1988-02-12), S. 783-785
Online
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Zugriff:
Mesoderm induction in the amphibian embryo can be studied by exposing animal region explants (destined to become ectoderm) to appropriate stimuli and assaying the appearance of mesodermal products like alpha-actin messenger RNA. Transforming growth factor beta 2 (TGF-beta 2), but not TGF-beta 1, was active in alpha-actin induction, while addition of fibroblast growth factor had a small synergistic effect. Medium conditioned by Xenopus XTC cells (XTC-CM), known to have powerful mesoderm-inducing activity, was shown to contain TGF-beta-like activity as measured by a radioreceptor binding assay, colony formation in NRK cells, and growth inhibition in CCL64 cells. The activity of XTC-CM in mesoderm induction and in growth inhibition of CCL64 cells was inhibited partially by antibodies to TGF-beta 2 but not by antibodies to TGF-beta 1. Thus, a TGF-beta 2-like molecule may be involved in mesoderm induction.
| Titel: |
Mesoderm Induction in Amphibians: the Role of TGF-β2-Like Factors
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| Autor/in / Beteiligte Person: | Roberts, Anita B. ; Dawid, Igor B. ; Danielpour, David ; Sporn, Michael B. ; Dart, Linda L. ; Rosa, Frédéric |
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| Zeitschrift: | Science, Jg. 239 (1988-02-12), S. 783-785 |
| Veröffentlichung: | American Association for the Advancement of Science (AAAS), 1988 |
| Medientyp: | unknown |
| ISSN: | 1095-9203 (print) ; 0036-8075 (print) |
| DOI: | 10.1126/science.3422517 |
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