Identification of splicing variants of Frabin with partly different functions and tissue distribution
In: Biochemical and biophysical research communications, Jg. 286 (2001-08-31), Heft 5
Online
unknown
Zugriff:
Frabin is a GDP/GTP exchange protein for Cdc42 small G protein with actin filament-binding activity. Frabin consists of the actin filament-binding domain, the Dbl homology domain, the first pleckstrin homology domain, the FYVE-finger domain, and the second pleckstrin homology domain in this order from the N-terminus. Frabin forms filopodia through direct activation of Cdc42 and lamellipodia through indirect activation of Rac small G protein. We isolated here two smaller splicing variants of frabin and named the original one, middle-size one, and smallest one frabin-alpha, -beta, and -gamma, respectively. Frabin-beta lacked the second pleckstrin homology domain and frabin-gamma lacked the FYVE-finger domain and the second pleckstrin homology domain. These three variants were expressed in all of the tissues examined but their expression levels are different depending on tissues. In L fibroblasts, all the three variants formed filopodia. As to lamellipodia, frabin-alpha formed them; frabin-beta formed them to a small extent; and frabin-gamma did not. In MDCK epithelial cells, frabin-alpha formed microspikes but frabin-beta or -gamma did not.
Titel: |
Identification of splicing variants of Frabin with partly different functions and tissue distribution
|
---|---|
Autor/in / Beteiligte Person: | Takekuni, Kyouji ; Nakanishi, Hiroyuki ; Takai, Yoshimi ; Ikeda, Wataru ; Itoh, Shinsuke |
Link: | |
Zeitschrift: | Biochemical and biophysical research communications, Jg. 286 (2001-08-31), Heft 5 |
Veröffentlichung: | 2001 |
Medientyp: | unknown |
ISSN: | 0006-291X (print) |
Schlagwort: |
|
Sonstiges: |
|