X chromosome aneuploidy in the Alzheimer’s disease brain
In: Molecular Cytogenetics, Jg. 7 (2014-03-06)
Online
unknown
Zugriff:
Background Although the link between brain aging and Alzheimer’s disease (AD) is a matter of debate, processes hallmarking cellular and tissue senescence have been repeatedly associated with its pathogenesis. Here, we have studied X chromosome aneuploidy (a recognized feature of aged cell populations) in the AD brain. Results Extended molecular neurocytogenetic analyses of X chromosome aneuploidy in 10 female AD as well as 10 age and sex matched female control postmortem brain samples was performed by multiprobe/quantitative FISH. Additionally, aneuploidy rate in the brain samples of 5 AD and as 5 age and sex matched control subjects were analyzed by interphase chromosome-specific multicolor banding (ICS-MCB). Totally, 182,500 cells in the AD brain and 182,500 cells in the unaffected brain were analyzed. The mean rate of X chromosome aneuploidy in AD samples was approximately two times higher than in control (control: mean - 1.32%, 95% CI 0.92- 1.71%; AD: mean - 2.79%, 95% CI 1.88-3.69; P = 0.013). One AD sample demonstrated mosaic aneuploidy of chromosome X confined to the hippocampus affecting about 10% of cells. ICS-MCB confirmed the presence of X chromosome aneuploidy in the hippocampal tissues of AD brain (control: mean - 1.74%, 95% CI 1.38- 2.10%; AD: mean - 4.92%, 95% CI 1.14-8.71; P
Titel: |
X chromosome aneuploidy in the Alzheimer’s disease brain
|
---|---|
Autor/in / Beteiligte Person: | Iourov, Ivan Y. ; Yurov, Yuri B. ; Liehr, Thomas ; Alexei D Kolotii ; Vorsanova, Svetlana G. |
Link: | |
Zeitschrift: | Molecular Cytogenetics, Jg. 7 (2014-03-06) |
Veröffentlichung: | Springer Science and Business Media LLC, 2014 |
Medientyp: | unknown |
ISSN: | 1755-8166 (print) |
DOI: | 10.1186/1755-8166-7-20 |
Schlagwort: |
|
Sonstiges: |
|