Excretion of alkaline phosphatase by Escherichia coli K-12 pho constitutive mutants transformed with plasmids carrying the alkaline phosphatase structural gene
In: Journal of Bacteriology, Jg. 164 (1985-12-01), S. 1376-1380
Online
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Zugriff:
Escherichia coli alkaline phosphatase constitutive mutants carrying a pst or a phoS mutation and a plasmid-bearing gene phoA+ excreted into the growth medium up to 50% of the total alkaline phosphatase production. This excretion was pH dependent and did not involve drastic modifications of the cell envelope. Alkaline phosphatase accounted for 80% of total released proteins. Amplification of gene phoA+ was a necessary condition for excretion to occur. When the beta-lactamase structural gene bla+ was coamplified with gene phoA+, both enzymes were excreted. pst-transformed excretory strains did not show the pleiotrophic phenotype previously described for lky mutants.
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Excretion of alkaline phosphatase by Escherichia coli K-12 pho constitutive mutants transformed with plasmids carrying the alkaline phosphatase structural gene
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Autor/in / Beteiligte Person: | J C Lazzaroni ; Portalier, Raymond ; Atlan, Danièle |
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Zeitschrift: | Journal of Bacteriology, Jg. 164 (1985-12-01), S. 1376-1380 |
Veröffentlichung: | American Society for Microbiology, 1985 |
Medientyp: | unknown |
ISSN: | 1098-5530 (print) ; 0021-9193 (print) |
DOI: | 10.1128/jb.164.3.1376-1380.1985 |
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