Detection of LOH of the RB1 gene in bladder cancers by PCR-RFLP

Açikbas, I. ; Kiliç, S. ; et al.

In: Urologia internationalis, Jg. 68 (2002-03-29), Heft 3

Online unknown

Zugriff:

Objectives: Retinoblastoma (RB1) gene involves in retinoblastoma, osteosarcoma, bladder, prostate, lung, breast carcinomas, and soft tissue sarcomas. Loss of heterozygosity (LOH) is the most common mutation of the gene. Methods: Xba I polymorphism in intron 17 of the gene was used to detect LOH in 20 bladder cancer patients. A cystitis and an osteosarcoma were used as control. LOH was investigated in three different kinds of samples (blood, paraffin-embedded tissue and fresh tissue) belonging to the same patients, and 20 blood samples, 20 paraffin-embedded tissue samples and 16 fresh tissue samples were obtained from 20 cancer patients. Results: None of the 20 blood samples showed LOH. Eleven out of 20 paraffin-embedded bladder tissues were amplified, 3 of them homozygous and all 8 informative paraffin-embedded tissues showed LOH. Five out of 16 fresh tumor tissues obtained were amplified, in 1 the fresh tissue was normal, 1 fresh tissue showed LOH and 3 were not digested by Xba I. Conclusion: The results of the study have suggested that detection of LOH of the RB1 gene by PCR-RFLP can be a good adjunctive test for evaluation of the bladder cancer.

Titel:	Detection of LOH of the RB1 gene in bladder cancers by PCR-RFLP
Autor/in / Beteiligte Person:	Açikbas, I. ; Kiliç, S. ; Keser, I. ; Karpuzoğlu, G. ; Bağci, H. ; Luleci, Guven
Link:	View record in OpenAIRE (Volltext) https://pubmed.ncbi.nlm.nih.gov/11919466
Zeitschrift:	Urologia internationalis, Jg. 68 (2002-03-29), Heft 3
Veröffentlichung:	2002
Medientyp:	unknown
ISSN:	0042-1138 (print)
Schlagwort:	bladder carcinoma Pathology gene amplification polymerase chain reaction Loss of Heterozygosity genetic analysis Polymerase Chain Reaction Loss of heterozygosity PCR-RFLP Bladder Neoplasm tumor suppressor gene Genes, Retinoblastoma restriction fragment length polymorphism Urinary bladder Retinoblastoma article Soft tissue medicine.anatomical_structure priority journal blood sampling cancer tissue Polymorphism, Restriction Fragment Length medicine.medical_specialty intron Urology PCR-LOH assay Fresh Tissue Bladder neoplasm osteosarcoma medicine Humans controlled study human Retinoblastoma gene cystitis neoplasms Carcinoma, Transitional Cell Bladder cancer business.industry heterozygosity loss Cancer medicine.disease major clinical study human tissue Urinary Bladder Neoplasms retinoblastoma gene bladder neoplasm Cancer research business Carcinoma, Transitional Cell/genetics Retinoblastoma/genetics Urinary Bladder Neoplasms/*genetics
Sonstiges:	Nachgewiesen in: OpenAIRE Rights: OPEN

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.