A roadmap to high‐resolution standard microcoil MAS NMR spectroscopy for metabolomics
In: NMR in Biomedicine NMR in Biomedicine, Wiley, 2022, pp.e4683. ⟨10.1002/nbm.4683⟩ NMR in Biomedicine, 2022, pp.e4683. ⟨10.1002/nbm.4683⟩; (2022)
Online
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Zugriff:
International audience; The current microcoil probe technology has emerged as a significant advancement in NMR applications to biological research of biofluids and continued to excel as a hyphenated tool with other prominent microdevices opening many new possibilities in multiple omics fields. However, this is contrary to biological samples like intact tissue or organism. This is due to the considerable challenges of incorporating the microcoil in a magic-angle spinning (MAS) probe without relinquishing the acquisition of high-resolution spectral data. Not until 2012, a microcoil MAS probe had shown promises on profiling the metabolome in submilligram tissue biopsy with spectral resolution on par with the conventional high-resolution MAS (HR-MAS) NMR. This result had subsequently triggered a great interest in the possibility of NMR analysis with microgram tissues and strived toward the probe development of 'high-resolution' capable microcoil MAS NMR spectroscopy. This review gives an overview of the issues and challenges in the probe development and summarizes the advancements toward metabolomics.
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A roadmap to high‐resolution standard microcoil MAS NMR spectroscopy for metabolomics
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| Autor/in / Beteiligte Person: | Wong, Alan ; Laboratoire Structure et Dynamique par Résonance Magnétique (LCF) (LSDRM) ; Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685) ; Institut Rayonnement Matière de Saclay (IRAMIS) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) ; ANR-16-CE11-0023,HRmicroMAS,SPECTROSCOPIE RMN MICROSCOPIC SPECIMENS(2016) ; ANR-12-JSV5-0005,HRMACS,Développement et exploration de nouveaux micro-détecteurs RMN tournants pour des analyses métabolomiques de biopsies de petite masse(2012) |
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| Quelle: | NMR in Biomedicine NMR in Biomedicine, Wiley, 2022, pp.e4683. ⟨10.1002/nbm.4683⟩ NMR in Biomedicine, 2022, pp.e4683. ⟨10.1002/nbm.4683⟩; (2022) |
| Veröffentlichung: | HAL CCSD, 2022 |
| Medientyp: | unknown |
| ISSN: | 0952-3480 (print) ; 1099-1492 (print) |
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