Apolipoprotein E Activates Akt Pathway in Neuro-2a in an Isoform-Specific Manner
In: Biochemical and Biophysical Research Communications, Jg. 292 (2002-03-01), S. 83-87
Online
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Zugriff:
Apolipoprotein E (apoE) is a ligand for members of the low density lipoprotein (LDL) receptor family, receptors highly expressed in neurons. A study of one of the mechanisms by which apoE might affect neuronal cell metabolism is reported herein. ApoE can induce Akt/protein kinase B phosphorylation in Neuro-2a via two different pathways. Both pathways are mediated by phosphatidylinositol 3-kinase and cAMP-dependent protein kinase. The first pathway is stimulated by apoE3 and E4, but not by E2, after a 1-h incubation. The process requires the binding of apoE to the heparan sulfate proteoglycan/LDL receptor-related protein complex. The second pathway is activated after a 2-h incubation of the cells, in another isoform-dependent manner (E2 = E3 dbl greater-than sign E4) and is mediated by calcium. Our results suggest that apoE might affect cell metabolism and survival in neurons in an isoform-specific manner by inducing novel signaling pathways.
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Apolipoprotein E Activates Akt Pathway in Neuro-2a in an Isoform-Specific Manner
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Autor/in / Beteiligte Person: | Visvikis, Sophie ; Shuvaev, Vladimir V. ; Taniguchi, Naoyuki ; Shibukawa, Yukinao ; Honke, Koichi ; Laffont, Isabelle ; Siest, Gérard ; Takahashi, Motoko |
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Zeitschrift: | Biochemical and Biophysical Research Communications, Jg. 292 (2002-03-01), S. 83-87 |
Veröffentlichung: | Elsevier BV, 2002 |
Medientyp: | unknown |
ISSN: | 0006-291X (print) |
DOI: | 10.1006/bbrc.2002.6586 |
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