Malate dehydrogenase activities are lower in some types of peripheral leucocytes of dogs and cats with type 1 diabetes mellitus
In: Research in Veterinary Science, Jg. 78 (2005-02-01), S. 39-44
Online
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Zugriff:
The activities of the enzymes in the malate-aspartate shuttle were measured in peripheral leucocytes of spontaneous type 1 diabetic dogs and cats treated with insulin injections. In the diabetic dogs and cats, fasting plasma glucose concentrations were three- or fourfold greater than the control levels in spite of insulin injections and the activities of cytosolic malate dehydrogenase (MDH), one of pivotal enzymes in the malate-aspartate shuttle, were remarkably lower than the controls. Depressed expression of cytosolic MDH mRNA was confirmed by RT-PCR analysis in the diabetic animals. The cytosolic ratio of MDH/lactate dehydrogenase (LDH) activity (M / L ratio) in leucocytes of the diabetic animals was significantly lower than that of normal control animals. The smaller M / L ratio appeared to reflect depression of energy metabolism in the diabetic animals. Intrinsically lower and further decreased MDH activities may be factors that induce insulin resistance observed in diabetic cats.
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Malate dehydrogenase activities are lower in some types of peripheral leucocytes of dogs and cats with type 1 diabetes mellitus
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Autor/in / Beteiligte Person: | Mizutani, H. ; Arai, Toshiro ; Fukuda, H. ; Tanaka, A. ; Sasaki, N. ; Magori, E. ; Kimura, N. ; Sako, Toshinori ; Nakamura, M. ; Inoue, A. |
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Zeitschrift: | Research in Veterinary Science, Jg. 78 (2005-02-01), S. 39-44 |
Veröffentlichung: | Elsevier BV, 2005 |
Medientyp: | unknown |
ISSN: | 0034-5288 (print) |
DOI: | 10.1016/j.rvsc.2004.06.001 |
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