High-level expression of rat D1A dopamine receptor cDNA in mouse fibroblast LTK- cells by n-butyrate
In: Clinical and experimental pharmacologyphysiology, Jg. 23 (1996-02-01), Heft 2
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Zugriff:
SUMMARY 1. In order to develop a simple, efficient system for the high-level expression of dopamine receptors in eukaryotic cells, we have studied the effects of n-butyrate on the expression of rat D1A dopamine receptor cDNA in mouse fibroblast LTK- cells as compared with those of n-butyrate on endogenous D1 receptor levels in opossum kidney cells. 2. In the transfected LTK- cell membranes with pRc/CMV-D1A receptor cDNA, a selective D1 dopamine antagonist, [3H]-SCH 23390, exhibited a K4 of 0.9 ± 0.1 nmol/L and a Bmax of 0.35 ± 0.05 pmol/mg protein (n= 5). 3. Addition of n-butyrate (2–10 mmol/L) to the culture medium for 48 h dose-dependently increased the D1A receptor level up to 1.5 ± 0.3 pmol/mg protein (n= 7), although the K4 values were not affected. The increase in receptor level was accompanied by an elevation of selective D1 agonist-induced adenylyl cyclase activity. 4. In contrast, n-butyrate treatment (2–10 mmol/L) did not affect either endogenous D1 receptor levels or fendoldopam-induced adenylyl cyclase activity in opossum kidney cells. 5. These results suggest n-butyrate is a useful tool for obtaining high-level expression of D1A dopamine receptor cDNA in mouse fibroblast LTK- cells.
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High-level expression of rat D1A dopamine receptor cDNA in mouse fibroblast LTK- cells by n-butyrate
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Autor/in / Beteiligte Person: | Horiuchi, Akira ; Felder, Robin A. |
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Zeitschrift: | Clinical and experimental pharmacologyphysiology, Jg. 23 (1996-02-01), Heft 2 |
Veröffentlichung: | 1996 |
Medientyp: | unknown |
ISSN: | 0305-1870 (print) |
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