Inhibition of CFU-GM by prostaglandins in a case of chronic T-cell lymphocytosis and neutropenia
In: British Journal of Haematology, Jg. 73 (1989-10-01), S. 148-151
Online
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Zugriff:
A 45-year-old Caucasian female with seropositive rheumatoid arthritis was found coincidentally to have a circulating lymphocytosis (6.4 x 10(9)/l) and neutropenia (0.1 x 10(9)/l). Initial presentation was with mouth ulceration and recurrent infections. A spleen scan showed no evidence of splenomegaly and serum titres against EBV, CMV and toxoplasmosis were negative. No anti-neutrophil antibodies were found. Marrow aspiration demonstrated a lymphocytosis of 60% with reduced numbers of granular precursors. Lymphocytes in both blood and bone marrow were CD3, CD8, CD16 and HLA-DR positive. Lymphocyte conditioned medium (LCM) generated from the patient's blood lymphocytes (without phytohaemagglutinin) was found to inhibit allogeneic colony-forming unit, granulocyte-macrophage (CFU-GM) stem cells in semi-solid culture compared with control LCM. This inhibitory activity was abrogated by the cytolytic removal of CD8 cells prior to LCM production and was significantly reduced by co-culture with indomethacin. Culture of the patient's marrow at autostimulatory light density marrow cell concentrations showed poor spontaneous CFU-GM colony formation until marrow CD8 lymphocytes were removed cytolytically. Prednisolone was used therapeutically (40 mg/d) and resulted in the patient's neutrophil count rising from 0.06 x 10(9)/l to 1.1 x 10(9)/l and a fall in the total lymphocyte count to 1.9 x 10(9)/l. Reevaluation of the patient's LCM post steroid therapy showed loss of the previous inhibitory effect. The patient's neutrophil count is maintained on oral azathioprine and indomethacin.
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Inhibition of CFU-GM by prostaglandins in a case of chronic T-cell lymphocytosis and neutropenia
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Autor/in / Beteiligte Person: | Smith, J. G. ; Maddison, P. J. ; James, I. ; Smith, M. A. ; Blundell, E. |
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Zeitschrift: | British Journal of Haematology, Jg. 73 (1989-10-01), S. 148-151 |
Veröffentlichung: | Wiley, 1989 |
Medientyp: | unknown |
ISSN: | 1365-2141 (print) ; 0007-1048 (print) |
DOI: | 10.1111/j.1365-2141.1989.tb00244.x |
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