Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function
In: Regenerative Therapy, Jg. 11 (2019-12-01), S. 131-138
Online
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Zugriff:
Introduction Macrophages play an important role in regulating inflammation and tissue regeneration. It is known that anti-inflammatory macrophages play an important role for tissue regeneration. The objective of this study is to modify macrophages phenotypes for anti-inflammatory function by utilizing drug delivery technology. Method In this study, 4 types of poly (L-lactic-co-glycolic acid) (PLGA) microspheres incorporating pioglitazone of an anti-inflammatory modifier (pio-MS) with different sizes were prepared. In vitro release test of pio-MS was performed in phosphate buffered-saline solution (PBS) containing 1 wt% of sodium lauryl sulfate. The arginase activity and the secretion of interleukin (IL)−10 as anti-inflammatory macrophage markers of mouse bone marrow derived-macrophages (BMDM) cultured with the pio-MS were evaluated. Results The sustained release of pioglitazone was observed from all types of pio-MS in vitro. When BMDM were cultured with the pio-MS with an average diameter of 40 μm (pio-MS40), the arginase activity and the secretion of IL-10 increased to a significant extent compared with other pio-MS. Conclusions The pio-MS40 with an diameter of 40 μm had a potential to induce the anti-inflammatory modification of BMDM in this culture system. The sustained release of pioglitazone is promoting to modify the macrophage function.
Highlights • Microspheres incorporating pioglitazone with different sizes were prepared. • Sustained release of pioglitazone from the microspheres were observed. • The effect of pioglitazone on macrophages was enhanced by the sustained release.
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Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function
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Autor/in / Beteiligte Person: | Tabata, Yasuhiko ; Jo, Jun-ichiro ; Momotori, Naoki |
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Zeitschrift: | Regenerative Therapy, Jg. 11 (2019-12-01), S. 131-138 |
Veröffentlichung: | Elsevier BV, 2019 |
Medientyp: | unknown |
ISSN: | 2352-3204 (print) |
DOI: | 10.1016/j.reth.2019.06.008 |
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