<scp>Lnc‐RNA LINC01279</scp>induces endometriosis via targeting of<scp>HOXA10</scp>
In: Journal of Obstetrics and Gynaecology Research, Jg. 47 (2021-03-03), S. 1825-1836
Online
unknown
Zugriff:
AIM To explore the regulatory role and molecular mechanism of lncRNA-LINC01279 in endometriosis (EMs). METHODS Between September 2018 and July 2019, 20 EMs patients and 20 healthy subjects were recruited to detect the expression of lncRNA-LINC01279 in EMs and in normal endometrium via qRT-PCR. Autograft was used to establish EMs models on Spraque-Dawley (SD) rats, which was followed by taking volume measurements of EMs endometrium and observing pathological changes in the morphology of EMs via hematoxylin and eosin (H&E) staining. The qRT-PCR technique was further carried out to determine mRNA expression of lncRNA-LINC01279 and HOXA10 in the serum of EMs rats and LINC01279 shRNA-transfected rats, while the protein expression of HOXA10 was determined using a Western blot. RESULTS EMs patients presented with upregulation of lncRNA-LINC01279 and downregulation of HOXA10 (p < 0.01 or 0.001). Online predictions further revealed that lncRNA-LINC01279 regulated the expression of HOXA10 via miRNA-135b. In EMs models, it was observed that there were a significantly enlarged endometrium and poor pathological morphology, significant upregulation of lncRNA-LINC01279, and downregulation of miR-135b and HOXA10 in serum (p < 0.05, 0.01 or 0.001). In the lncRNA-LINC01279 shRNA group, EMs rats, following treatment, had a sharp decrease in the volume of EMs endometrium, and an improvement in pathological morphology, while lncRNA-LINC01279 was downregulated, with upregulation of miR-135b and HOXA10 (p < 0.05, 0.01 or 0.001). CONCLUSION LncRNA-LINC01279, by the mechanism of targeting miR-135b, has the potential to downregulate the expression of HOXA10, and therefore, can promote the development and progression of EMs.
Titel: |
<scp>Lnc‐RNA LINC01279</scp>induces endometriosis via targeting of<scp>HOXA10</scp>
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Autor/in / Beteiligte Person: | Wei, Fu ; Yang, Wenmei ; Liu, Liling ; Cheng, Junping ; Zhi, Zhifu ; Tan, Weihong ; Pang, Lihong |
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Zeitschrift: | Journal of Obstetrics and Gynaecology Research, Jg. 47 (2021-03-03), S. 1825-1836 |
Veröffentlichung: | Wiley, 2021 |
Medientyp: | unknown |
ISSN: | 1447-0756 (print) ; 1341-8076 (print) |
DOI: | 10.1111/jog.14723 |
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