Novel multiplex bead-based assay with LNA-modified probes for detection of MPL exon 10 mutations
In: Leukemia Research, Jg. 35 (2011-08-01), S. 1120-1123
Online
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Zugriff:
MPL exon 10 mutations were the second class of mutations shown to be associated with the pathogenesis of some Philadelphia chromosome – negative myeloproliferative neoplasms (MPNs). Recently, their identification gained wide recognition in the diagnostic work-up for suspected cases of JAK2 V617F negative MPNs. Various molecular approaches have been applied, yet universally accepted method is still lacking. We aimed at development and validation of a novel bead-based liquid assay using Locked nucleic acids (LNA)-modified oligonucleotide probes for multiplexed detection of the following MPL mutations: W515L/K/A/R. Testing on both artificial plasmid constructs and on clinical samples revealed that the method was comparable in terms of specificity to direct sequencing and had a much higher sensitivity of 1% mutant alleles. This method could be successfully implemented in the diagnostic work-up for MPNs. Furthermore, this system allows further multiplexing for single-tube identification of different mutations associated with MPNs.
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Novel multiplex bead-based assay with LNA-modified probes for detection of MPL exon 10 mutations
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Autor/in / Beteiligte Person: | Shivarov, Velizar ; Ivanova, Milena ; Hadjiev, Evgueniy ; Naumova, Elissaveta |
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Zeitschrift: | Leukemia Research, Jg. 35 (2011-08-01), S. 1120-1123 |
Veröffentlichung: | Elsevier BV, 2011 |
Medientyp: | unknown |
ISSN: | 0145-2126 (print) |
DOI: | 10.1016/j.leukres.2011.04.012 |
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