TheMycobacterium tuberculosisrecombinant LprN protein ofmce4operon induces Th-1 type response deleterious to protection in mice
In: Pathogens and Disease, 2014-07-01
Online
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Zugriff:
Lipoproteins are known to be effective immunogens and affect both innate and adaptive immunity. The lprN gene of Mycobacterium tuberculosis has been predicted to encode for a putative lipoprotein in silico. Here, we studied its function as an immunogen by in vivo studies in mice. The recombinant LprN protein, expressed and purified in Escherichia coli, triggered a cell-mediated immune response in BALB/c mice. This was observed by significantly higher T-cell proliferation and increased production of TNF-α and IFN-γ cytokines. However, pre-exposure to LprN protein failed to provide protection in mice after challenge with a virulent strain of M. tuberculosis. Histological examination showed an increase in tissue destruction in experimental animals, indicating an immunogenic potential for LprN protein that enhanced the virulence of bacilli.
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TheMycobacterium tuberculosisrecombinant LprN protein ofmce4operon induces Th-1 type response deleterious to protection in mice
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Autor/in / Beteiligte Person: | Saini, Neeraj K. ; Sinha, Rajesh ; Bose, Mridula ; Pasricha, Rashmi ; Brahmachari, Vani ; Rathor, Nisha ; Mishra, Kiran ; Varma-Basil, Mandira ; Pathak, Rakesh |
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Zeitschrift: | Pathogens and Disease, 2014-07-01 |
Veröffentlichung: | Oxford University Press (OUP), 2014 |
Medientyp: | unknown |
ISSN: | 2049-632X (print) |
DOI: | 10.1111/2049-632x.12200 |
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