Association of post-thaw viable CD34+ cells and CFU-GM with time to hematopoietic engraftment
In: Bone Marrow Transplantation, Jg. 35 (2005-03-21), S. 881-887
Online
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Zugriff:
In all, 78 peripheral hematopoietic progenitor cell collections from 52 patients were evaluated using our previously published validated post-thaw assays at the time of collection and following transplantation by assessment of viable CD34(+) cells, and granulocyte-macrophage colony-forming units (CFU-GM) cryopreserved in quality control vials. The median (range) post-thaw recovery of viable CD34(+) cells and CFU-GM was 66.4% (36.1-93.6%) and 63.0% (28.6-85.7%), respectively, which did not show significant correlation with the engraftment of either neutrophils (P=0.136 and 0.417, respectively) or platelets (P=0.88 and 0.126, respectively). However, the reinfused viable CD34(+) cells/kg of patient weight pre- or post-cryopreservation showed significant correlation to engraftment of neutrophils (P=0.0001 and 0.001, respectively) and platelets (P=0.023 and 0.010, respectively), whereas CFU-GM pre- or post-cryopreservation was significantly correlated to neutrophils (P=0.011 and 0.007, respectively) but not to platelets (P=0.112 and 0.100, respectively). The results show that post-cryopreservation assessment of viable CD34(+) cells or CFU-GM is as reliable a predictor of rapid engraftment as that of pre-cryopreservation measures. Therefore, the post-cryopreservation number of viable CD34(+) cells or CFU-GM should be used to eliminate the risks of unforeseen cell loss that could occur during cryopreservation or long-term storage.
Titel: |
Association of post-thaw viable CD34+ cells and CFU-GM with time to hematopoietic engraftment
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Autor/in / Beteiligte Person: | Letcher, B ; Cabuhat, M ; McGann, Locksley E. ; Larratt, Loree ; Yang, H ; Acker, Jason P. |
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Zeitschrift: | Bone Marrow Transplantation, Jg. 35 (2005-03-21), S. 881-887 |
Veröffentlichung: | Springer Science and Business Media LLC, 2005 |
Medientyp: | unknown |
ISSN: | 1476-5365 (print) ; 0268-3369 (print) |
DOI: | 10.1038/sj.bmt.1704926 |
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