In Vitro and In Vivo Activation Induces BAFF and APRIL Expression in B Cells
In: The Journal of Immunology, Jg. 179 (2007-11-01), S. 5947-5957
Online
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Zugriff:
B cell-activating factor (BAFF) and a proliferation-inducing ligand (APRIL) play key roles in peripheral B cell survival, maturation, and differentiation. BAFF and APRIL are produced by a variety of cell types such as macrophages/monocytes and dendritic cells. Our analysis shows that BAFF mRNA is also expressed in all B cell subsets isolated from bone marrow, spleen, and peritoneal cavity of BALB/c mice. APRIL expression is restricted to early stages of B cell development in the bone marrow and the peritoneal B1 subset. Stimulation of B2 and B1 cells with LPS or CpG-oligodeoxynucleotides induced MyD88-dependent plasma cell differentiation and intracellular expression of BAFF and APRIL. Furthermore, activation of B cells up-regulated membrane expression of BAFF. The finding that in vitro activation of B cells is inhibited by the antagonist transmembrane activator and calcium modulator ligand interactor Ig, indicates that BAFF and/or APRIL are released into the culture supernatants. It shows that B cell survival, proliferation, and differentiation are supported by an autocrine pathway. In vivo activation of B cells with a T-dependent Ag- induced BAFF expression in germinal center B cells. In (NZB × NZW)F1 mice with established autoimmune disease, marginal zone, germinal center B cells, as well as splenic plasma cells expressed high levels of BAFF. In (NZB × NZW)F1 mice, the continuous activation of B cells and thus overexpression of BAFF and APRIL may contribute to the development of autoimmune disease.
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In Vitro and In Vivo Activation Induces BAFF and APRIL Expression in B Cells
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Autor/in / Beteiligte Person: | Enghard, Philipp ; Berek, Claudia ; Van Trung Chu ; Riemekasten, Gabriela |
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Zeitschrift: | The Journal of Immunology, Jg. 179 (2007-11-01), S. 5947-5957 |
Veröffentlichung: | The American Association of Immunologists, 2007 |
Medientyp: | unknown |
ISSN: | 1550-6606 (print) ; 0022-1767 (print) |
DOI: | 10.4049/jimmunol.179.9.5947 |
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