Internalization of Rat FSH and LH/CG Receptors by rec-eCG in CHO-K1 Cells
In: Development & Reproduction, Jg. 21 (2017-06-01), S. 111-120
Online
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Zugriff:
Equine chorionic gonadotropin (eCG) is a unique molecule that elicits the response characteristics of both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in other species. Previous studies from this laboratory had demonstrated that recombinant eCG (rec-eCG) from Chinese hamster ovary (CHO-K1) cells exhibited both FSH- and LH-like activity in rat granulosa and Leydig cells. In this study, we analyzed receptor internalization through rec-eCGs, wild type eCG (eCGβ/α) and mutant eCG (eCGβ/αΔ56) with an N-linked oligosaccharide at Asn56 of the α-subunit. Both the rec-eCGs were obtained from CHO-K1 cells. The agonist activation of receptors was analyzed by measuring stimulation time and concentrations of rec-eCGs. Internalization values in the stably selected rat follicle-stimulating hormone receptor (rFSHR) and rat luteinizing/chorionic gonadotropin receptor (rLH/CGR) were highest at 50 min after stimulation with 10 ng of rec-eCGβ/α. The dose-dependent response was highest when 10 ng of rec-eCGβ/α was used. The deglycosylated eCGβ/αΔ56 mutant did not enhance the agonist-stimulated internalization. We concluded that the state of activation of rFSHR and rLH/CGR could be modulated through agonist-stimulated internalization. Our results suggested that the eLH/CGRs are mostly internalized within 60 min by agonist-stimulation by rec-eCG. We also suggested that the lack of responsiveness of the deglycosylated eCGβ/ αΔ56 was likely because the site of glycosylation played a pivotal role in agonist-stimulated internalization in cells expressing rFSHR and rLH/CGR.
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Internalization of Rat FSH and LH/CG Receptors by rec-eCG in CHO-K1 Cells
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Autor/in / Beteiligte Person: | Seong, Hun-Ki ; Min, Kwan-Sik ; Kang, Myung-Hwa ; Park, Jong-Ju ; Munkhzaya, Byambaragchaa ; Kim, Jeong-Soo |
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Zeitschrift: | Development & Reproduction, Jg. 21 (2017-06-01), S. 111-120 |
Veröffentlichung: | Korean Society of Developmental Biology, 2017 |
Medientyp: | unknown |
ISSN: | 2465-9541 (print) ; 2465-9525 (print) |
DOI: | 10.12717/dr.2017.21.2.111 |
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